PMID- 18597347
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20100520
LR  - 20080703
IS  - 0006-3592 (Print)
IS  - 0006-3592 (Linking)
VI  - 37
IP  - 2
DP  - 1991 Jan 20
TI  - Continuous cell suspension processing using magnetically stabilized fluidized 
      beds.
PG  - 110-20
AB  - The behavior of a cell suspension in a continuous magnetically stabilized 
      fluidized bed (MSFB) was investigated both experimentally and theoretically. The 
      low, constant pressure drop and fluidity of the solids phase in the MSFB allowed 
      a continuous countercurrent separator to be constructed. The magnetic field 
      eliminated all motion of the solids phase (nickel spheres) and produced a device 
      similar to a packed-bed depth filter. Yeast cells were used as the suspended 
      solids and the performance of the MSFB filter was assessed as a function of the 
      bed height, solids velocity, cell concentration, and liquid composition. Removal 
      rates could be adjusted by controlling the cell/support interaction and were 
      found to be as high as 99%. A mathematical model was used to aid in understanding 
      this filtration and was found to agree qualitatively with all experimental 
      observations. Comparison of the model with the data suggests that both cell/cell 
      binding and cell shadowing are occurring.
FAU - Terranova, B E
AU  - Terranova BE
AD  - Genetics Institute, Andover, Massachusetts, 01003, USA.
FAU - Burns, M A
AU  - Burns MA
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biotechnol Bioeng
JT  - Biotechnology and bioengineering
JID - 7502021
EDAT- 1991/01/20 00:00
MHDA- 1991/01/20 00:01
CRDT- 1991/01/20 00:00
PHST- 1991/01/20 00:00 [pubmed]
PHST- 1991/01/20 00:01 [medline]
PHST- 1991/01/20 00:00 [entrez]
AID - 10.1002/bit.260370204 [doi]
PST - ppublish
SO  - Biotechnol Bioeng. 1991 Jan 20;37(2):110-20. doi: 10.1002/bit.260370204.