PMID- 18597617
OWN - NLM
STAT- MEDLINE
DCOM- 20090217
LR  - 20220317
IS  - 1557-8534 (Electronic)
IS  - 1547-3287 (Print)
IS  - 1547-3287 (Linking)
VI  - 17
IP  - 6
DP  - 2008 Dec
TI  - Defining stem and progenitor cells within adipose tissue.
PG  - 1053-63
LID - 10.1089/scd.2008.0117 [doi]
AB  - Adipose tissue-derived stem cells (ADSC) are routinely isolated from the stromal 
      vascular fraction (SVF) of homogenized adipose tissue. Freshly isolated ADSC 
      display surface markers that differ from those of cultured ADSC, but both cell 
      preparations are capable of multipotential differentiation. Recent studies have 
      inferred that these progenitors may reside in a perivascular location where they 
      appeared to coexpress CD34 and smooth muscle actin (alpha-SMA) but not CD31. 
      However, these studies provided only limited histological evidence to support 
      such assertions. In the present study, we employed immunohistochemistry and 
      immunofluorescence to define more precisely the location of ADSC within human 
      adipose tissue. Our results show that alpha-SMA and CD31 localized within smooth 
      muscle and endothelial cells, respectively, in all blood vessels examined. CD34 
      localized to both the intima (endothelium) and adventitia neither of which 
      expressed alpha-SMA. The niche marker Wnt5a was confined exclusively to the 
      vascular wall within mural smooth muscle cells. Surprisingly, the widely accepted 
      mesenchymal stem cell marker STRO-1 was expressed exclusively in the endothelium 
      of capillaries and arterioles but not in the endothelium of arteries. The 
      embryonic stem cell marker SSEA1 localized to a pericytic location in capillaries 
      and in certain smooth muscle cells of arterioles. Cells expressing the embryonic 
      stem cell markers telomerase and OCT4 were rare and observed only in capillaries. 
      Based on these findings and evidence gathered from the existing literature, we 
      propose that ADSC are vascular precursor (stem) cells at various stages of 
      differentiation. In their native tissue, ADSC at early stages of differentiation 
      can differentiate into tissue-specific cells such as adipocytes. Isolated, ADSC 
      can be induced to differentiate into additional cell types such as osteoblasts 
      and chondrocytes.
FAU - Lin, Guiting
AU  - Lin G
AD  - Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, 
      University of California, San Francisco, California 94143-0738, USA.
FAU - Garcia, Maurice
AU  - Garcia M
FAU - Ning, Hongxiu
AU  - Ning H
FAU - Banie, Lia
AU  - Banie L
FAU - Guo, Ying-Lu
AU  - Guo YL
FAU - Lue, Tom F
AU  - Lue TF
FAU - Lin, Ching-Shwun
AU  - Lin CS
LA  - eng
GR  - P50 DK064538/DK/NIDDK NIH HHS/United States
GR  - P50 DK064538-010002/DK/NIDDK NIH HHS/United States
GR  - R01 DK069655/DK/NIDDK NIH HHS/United States
GR  - R01 DK069655-05/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Stem Cells Dev
JT  - Stem cells and development
JID - 101197107
RN  - 0 (Antigens, Differentiation)
SB  - IM
MH  - Adipose Tissue/*cytology/*metabolism
MH  - Adult
MH  - Antigens, Differentiation/*metabolism
MH  - Cell Differentiation/*physiology
MH  - Female
MH  - Humans
MH  - Male
MH  - Multipotent Stem Cells/*cytology/*metabolism
MH  - Organ Specificity/physiology
PMC - PMC2865901
MID - NIHMS196751
EDAT- 2008/07/04 09:00
MHDA- 2009/02/20 09:00
PMCR- 2009/12/01
CRDT- 2008/07/04 09:00
PHST- 2008/07/04 09:00 [pubmed]
PHST- 2009/02/20 09:00 [medline]
PHST- 2008/07/04 09:00 [entrez]
PHST- 2009/12/01 00:00 [pmc-release]
AID - 10.1089/scd.2008.0117 [pii]
AID - 10.1089/scd.2008.0117 [doi]
PST - ppublish
SO  - Stem Cells Dev. 2008 Dec;17(6):1053-63. doi: 10.1089/scd.2008.0117.