PMID- 18597806
OWN - NLM
STAT- MEDLINE
DCOM- 20080923
LR  - 20080818
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Linking)
VI  - 378
IP  - 2
DP  - 2008 Sep 1
TI  - C-type lectin Mermaid inhibits dendritic cell mediated HIV-1 transmission to CD4+ 
      T cells.
PG  - 323-8
LID - 10.1016/j.virol.2008.05.025 [doi]
AB  - Dendritic cells (DCs) are important in HIV-1 transmission; DCs capture invading 
      HIV-1 through the interaction of the gp120 oligosaccharides with the C-type 
      lectin DC-SIGN and migrate to the lymphoid tissues where HIV-1 is transmitted to 
      T cells. Thus, the HIV-1 envelope glycoprotein gp120 is an attractive target to 
      prevent interactions with DCs and subsequent viral transmission. Here, we have 
      investigated whether the structural homologue of DC-SIGN, the nematode C-type 
      lectin Mermaid can be used to prevent HIV-1 transmission by DCs. Our data 
      demonstrate that Mermaid interacts with high mannose structures present on HIV-1 
      gp120 and thereby inhibits HIV-1 binding to DC-SIGN on DCs. Moreover, Mermaid 
      inhibits DC-SIGN-mediated HIV-1 transmission from DC to T cells. We have 
      identified Mermaid as a non-cytotoxic agent that shares the glycan specificity 
      with DC-SIGN and inhibits DC-SIGN-gp120 interaction. The results are important 
      for the anti-HIV-1 microbicide development directed at preventing DC-HIV-1 
      interactions.
FAU - Nabatov, Alexey A
AU  - Nabatov AA
AD  - Department of Molecular Cellular Biology and Immunology, VU University Medical 
      Center, vd Boechorststraat 7, 1081 BT, Amsterdam, The Netherlands.
FAU - de Jong, Marein A W P
AU  - de Jong MA
FAU - de Witte, Lot
AU  - de Witte L
FAU - Bulgheresi, Silvia
AU  - Bulgheresi S
FAU - Geijtenbeek, Teunis B H
AU  - Geijtenbeek TB
LA  - eng
GR  - HHSN266200400091C/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20080701
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Anti-HIV Agents)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (DC-specific ICAM-3 grabbing nonintegrin)
RN  - 0 (HIV Envelope Protein gp120)
RN  - 0 (Helminth Proteins)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Receptors, Cell Surface)
SB  - IM
MH  - Animals
MH  - Anti-HIV Agents/*pharmacology
MH  - CD4-Positive T-Lymphocytes/*virology
MH  - Cell Adhesion Molecules/metabolism
MH  - Cell Line
MH  - Cells, Cultured
MH  - Dendritic Cells/*virology
MH  - HIV Envelope Protein gp120/metabolism
MH  - HIV-1/*drug effects
MH  - Helminth Proteins/*pharmacology/toxicity
MH  - Humans
MH  - Lectins, C-Type/*metabolism
MH  - Nematoda
MH  - Protein Binding
MH  - Receptors, Cell Surface/metabolism
MH  - Virus Attachment
EDAT- 2008/07/04 09:00
MHDA- 2008/09/24 09:00
CRDT- 2008/07/04 09:00
PHST- 2008/04/04 00:00 [received]
PHST- 2008/04/25 00:00 [revised]
PHST- 2008/05/22 00:00 [accepted]
PHST- 2008/07/04 09:00 [pubmed]
PHST- 2008/09/24 09:00 [medline]
PHST- 2008/07/04 09:00 [entrez]
AID - S0042-6822(08)00365-6 [pii]
AID - 10.1016/j.virol.2008.05.025 [doi]
PST - ppublish
SO  - Virology. 2008 Sep 1;378(2):323-8. doi: 10.1016/j.virol.2008.05.025. Epub 2008 
      Jul 1.