PMID- 18598206
OWN - NLM
STAT- MEDLINE
DCOM- 20081106
LR  - 20161018
IS  - 1434-6621 (Print)
IS  - 1434-6621 (Linking)
VI  - 46
IP  - 5
DP  - 2008
TI  - Determination of reference intervals for 26 commonly measured biochemical 
      analytes with consideration of long-term within-individual variation.
PG  - 691-8
LID - 10.1515/CCLM.2008.140 [doi]
AB  - BACKGROUND: A reference interval (RI) is usually derived from a data set obtained 
      cross-sectionally without consideration of the long-term analytical coefficient 
      of variation (CV) (CV(A)) or within-individual CV (CV1). METHODS: A total of 135 
      healthy volunteers, 22-59 years of age, working in eight clinical laboratories in 
      Japan participated in a study to determine RIs for 26 commonly measured 
      biochemical analytes. Blood samples were collected monthly for 1 year and 
      measured in each laboratory. Common quality control (QC) specimens were measured 
      monthly. RIs were derived parametrically using a modified Box-Cox power 
      transformation method. Subjects with abnormal results for test items other than 
      the one for derivation were excluded. RESULTS: Although there was no 
      statistically significant between-month variation, unacceptably large 
      between-laboratory differences were observed in QF test results. Thus, all values 
      were transformed to those of the main laboratory based on cross-check test 
      results. CV1 values computed by two-level nested analysis of variance were 
      generally greater than those commonly cited in the literature. Average RIs 
      derived from the monthly results agreed well with those derived from the entire 
      results. However, CVs of lower and upper limits of the monthly RIs were greater 
      than CV(A). CONCLUSIONS: To derive reliable RIs, it is important to consider 
      long-term variability of CV1 and CV(A).
FAU - Matsubara, Akemi
AU  - Matsubara A
AD  - 1Clinical Laboratory, Hiroshima University Hospital, Hiroshima, Japan.
FAU - Ichihara, Kiyoshi
AU  - Ichihara K
FAU - Fukutani, Shunji
AU  - Fukutani S
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Clin Chem Lab Med
JT  - Clinical chemistry and laboratory medicine
JID - 9806306
SB  - IM
MH  - Adult
MH  - Blood Chemical Analysis/*statistics & numerical data
MH  - Data Interpretation, Statistical
MH  - Female
MH  - Humans
MH  - Japan
MH  - Male
MH  - Middle Aged
MH  - Reference Values
MH  - Reproducibility of Results
MH  - Time
EDAT- 2008/07/05 09:00
MHDA- 2008/11/07 09:00
CRDT- 2008/07/05 09:00
PHST- 2008/07/05 09:00 [pubmed]
PHST- 2008/11/07 09:00 [medline]
PHST- 2008/07/05 09:00 [entrez]
AID - 10.1515/CCLM.2008.140 [pii]
AID - 10.1515/CCLM.2008.140 [doi]
PST - ppublish
SO  - Clin Chem Lab Med. 2008;46(5):691-8. doi: 10.1515/CCLM.2008.140.