PMID- 18599811
OWN - NLM
STAT- MEDLINE
DCOM- 20090211
LR  - 20080918
IS  - 1549-4918 (Electronic)
IS  - 1066-5099 (Linking)
VI  - 26
IP  - 9
DP  - 2008 Sep
TI  - Intra-bone marrow injection of donor bone marrow cells suspended in collagen gel 
      retains injected cells in bone marrow, resulting in rapid hemopoietic recovery in 
      mice.
PG  - 2211-6
LID - 10.1634/stemcells.2008-0035 [doi]
AB  - We have recently developed an innovative bone marrow transplantation (BMT) 
      method, intra-bone marrow (IBM)-BMT, in which donor bone marrow cells (BMCs) are 
      injected directly into the recipient bone marrow (BM), resulting in the rapid 
      recovery of donor hemopoiesis and permitting a reduction in radiation doses as a 
      pretreatment for BMT. However, even with this IBM injection, some of the injected 
      BMCs were found to enter into circulation. Therefore, we attempted to modify the 
      method to allow the efficient retention of injected BMCs in the donor BM. The 
      BMCs of enhanced green fluorescent protein transgenic mice (C57BL/6 background) 
      were suspended in collagen gel (CG) or phosphate-buffered saline (PBS), and these 
      cells were then injected into the BM of irradiated C57BL/6 mice. The numbers of 
      retained donor cells in the injected BM, the day 12 colony-forming units of 
      spleen (CFU-S) counts, and the reconstitution of donor cells after IBM-BMT were 
      compared between the CG and PBS groups. The number of transplanted cells detected 
      in the injected BM in the CG group was significantly higher than that in the PBS 
      group. We next carried out CFU-S assays. The spleens of mice in the CG group 
      showed heavier spleen weight and considerably higher CFU-S counts than in the PBS 
      group. Excellent reconstitution of donor hemopoietic cells in the CG group was 
      observed in the long term (>100 days). These results suggest that the IBM 
      injection of BMCs suspended in CG is superior to the injection of BMCs suspended 
      in PBS.
FAU - Shi, Ming
AU  - Shi M
AD  - First Department of Pathology, Kansai Medical University, 10-15 Fumizono-cho, 
      Moriguchi City, Japan.
FAU - Adachi, Yasushi
AU  - Adachi Y
FAU - Shigematsu, Akio
AU  - Shigematsu A
FAU - Koike-Kiriyama, Naoko
AU  - Koike-Kiriyama N
FAU - Feng, Wei
AU  - Feng W
FAU - Yanai, Seji
AU  - Yanai S
FAU - Yunze, Cui
AU  - Yunze C
FAU - Lian, Zhe-Xiong
AU  - Lian ZX
FAU - Li, Juan
AU  - Li J
FAU - Ikehara, Susumu
AU  - Ikehara S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080703
PL  - England
TA  - Stem Cells
JT  - Stem cells (Dayton, Ohio)
JID - 9304532
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - Bone Marrow
MH  - Bone Marrow Cells/*cytology
MH  - Bone Marrow Transplantation/*methods
MH  - Collagen
MH  - Colony-Forming Units Assay
MH  - Injections
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Spleen/cytology
MH  - Whole-Body Irradiation
EDAT- 2008/07/05 09:00
MHDA- 2009/02/12 09:00
CRDT- 2008/07/05 09:00
PHST- 2008/07/05 09:00 [pubmed]
PHST- 2009/02/12 09:00 [medline]
PHST- 2008/07/05 09:00 [entrez]
AID - 2008-0035 [pii]
AID - 10.1634/stemcells.2008-0035 [doi]
PST - ppublish
SO  - Stem Cells. 2008 Sep;26(9):2211-6. doi: 10.1634/stemcells.2008-0035. Epub 2008 
      Jul 3.