PMID- 18600179
OWN - NLM
STAT- MEDLINE
DCOM- 20090407
LR  - 20080724
IS  - 1537-4513 (Electronic)
IS  - 1524-9557 (Linking)
VI  - 31
IP  - 7
DP  - 2008 Sep
TI  - Minor role of bystander tolerance to fetal calf serum in a peptide-specific 
      dendritic cell vaccine model against autoimmunity: comparison with serum-free 
      cultures.
PG  - 656-64
LID - 10.1097/CJI.0b013e31818283ef [doi]
AB  - Dendritic cells (DCs) are currently considered as promising tools for vaccination 
      against tumors and also autoimmune responses. A major point of concern has been 
      the use of fetal calf serum (FCS) as a source of heterologous antigen in DC 
      cultures. FCS peptides can be presented by the DCs and cause T-cell responses in 
      the recipient. We investigated the role of FCS in an autoimmune model where DC 
      injections can prevent peptide-specifically from experimental autoimmune 
      encephalomyelitis (EAE). We show that murine bone marrow-derived DCs generated in 
      FCS-containing or serum-free media resulting in a similar phenotype, maturation 
      potential, and functions. Peptide-specific protection could be achieved similarly 
      with FCS-DC or serum-free DCs. Although FCS-DC induced strong CD4 T cell 
      proliferation and cytokine production against FCS, these T cells lack antigenic 
      recall during EAE. Even if FCS was reinjected, the effect on EAE resulted only in 
      a 3-day delay of disease onset. Together, our data show that presentation of 
      bystander antigens by peptide-specific DC vaccinations may have little influence 
      on T-cell responses in vivo if the bystander antigen cannot be recalled by 
      specific T cells.
FAU - Roner, Susanne
AU  - Roner S
AD  - Department of Dermatology, University Hospital Erlangen, Erlangen, Germany.
FAU - Zinser, Elisabeth
AU  - Zinser E
FAU - Menges, Mauritius
AU  - Menges M
FAU - Wiethe, Carsten
AU  - Wiethe C
FAU - Littmann, Leonie
AU  - Littmann L
FAU - Hanig, Jens
AU  - Hanig J
FAU - Steinkasserer, Alexander
AU  - Steinkasserer A
FAU - Lutz, Manfred B
AU  - Lutz MB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunother
JT  - Journal of immunotherapy (Hagerstown, Md. : 1997)
JID - 9706083
RN  - 0 (Blood Proteins)
RN  - 0 (Culture Media, Serum-Free)
RN  - 0 (Peptides)
SB  - IM
MH  - Animals
MH  - Antigen Presentation/*immunology
MH  - Blood Proteins/immunology
MH  - CD4-Positive T-Lymphocytes/cytology/immunology/metabolism
MH  - Cattle
MH  - Cell Differentiation/*immunology
MH  - Cell Proliferation/drug effects
MH  - Culture Media, Serum-Free
MH  - Dendritic Cells/*immunology
MH  - Encephalomyelitis, Autoimmune, Experimental/immunology/*prevention & control
MH  - Lymphocyte Activation/*immunology
MH  - Mice
MH  - Peptides/immunology
MH  - T-Cell Antigen Receptor Specificity/drug effects/immunology
MH  - *Vaccination
EDAT- 2008/07/05 09:00
MHDA- 2009/04/08 09:00
CRDT- 2008/07/05 09:00
PHST- 2008/07/05 09:00 [pubmed]
PHST- 2009/04/08 09:00 [medline]
PHST- 2008/07/05 09:00 [entrez]
AID - 00002371-200809000-00006 [pii]
AID - 10.1097/CJI.0b013e31818283ef [doi]
PST - ppublish
SO  - J Immunother. 2008 Sep;31(7):656-64. doi: 10.1097/CJI.0b013e31818283ef.