PMID- 18600858
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20091214
LR  - 20080708
IS  - 0006-3592 (Print)
IS  - 0006-3592 (Linking)
VI  - 38
IP  - 9
DP  - 1991 Nov
TI  - Continuous protein separations in a magnetically stabilized fluidized bed using 
      nonmagnetic supports.
PG  - 963-71
AB  - Continuous protein separations were performed using a magnetically stabilized 
      fluidized bed (MSFB) and a commercially available affinity adsorption resin that 
      contained no magnetically susceptible material. These nonmagnetic materials can 
      be stabilized at relatively low fields (<75 G requiring <30 W) if sufficient 
      magnetically susceptible particles are also present in the stabilized bed. The 
      minimum amount of magnetic particles necessary to stabilize the bed is as low as 
      20% by volume and is a function of various parameters including the size and 
      density of both particles, the magnetic field strength, and the fluidization 
      velocity. Advantages of these beds for performing separations include true 
      continuous, countercurrent liquid-solids contact, mass-transfer efficiencies 
      nearly equal to that of packed beds, and the ability of handle suspended cells or 
      cell debris. A variety of commercially available affinity, ion-exchange, and 
      adsorptive supports can be used in the bed for continuous separations; results 
      are presented for the adsorption and recovery of lysozyme from an aqueous mixture 
      of lysozyme and myoglobin using an affinity resin.
FAU - Chetty, A S
AU  - Chetty AS
AD  - E.I. DuPont Engineering Department, Newark, DE 19714-6090, USA.
FAU - Burns, M A
AU  - Burns MA
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biotechnol Bioeng
JT  - Biotechnology and bioengineering
JID - 7502021
EDAT- 1991/11/01 00:00
MHDA- 1991/11/01 00:01
CRDT- 1991/11/01 00:00
PHST- 1991/11/01 00:00 [pubmed]
PHST- 1991/11/01 00:01 [medline]
PHST- 1991/11/01 00:00 [entrez]
AID - 10.1002/bit.260380902 [doi]
PST - ppublish
SO  - Biotechnol Bioeng. 1991 Nov;38(9):963-71. doi: 10.1002/bit.260380902.