PMID- 18601743
OWN - NLM
STAT- MEDLINE
DCOM- 20080825
LR  - 20211020
IS  - 1471-2202 (Electronic)
IS  - 1471-2202 (Linking)
VI  - 9
DP  - 2008 Jul 5
TI  - Time-dependent biphasic modulation of human BDNF by antidepressants in 
      neuroblastoma cells.
PG  - 61
LID - 10.1186/1471-2202-9-61 [doi]
AB  - BACKGROUND: Recent rodent studies reported that antidepressant treatments affect 
      the expression of brain-derived neurotrophic factor (BDNF) mRNA in a way that is 
      dependent on treatment duration, by selective modulation of different BDNF 
      transcripts. However, no data are available for the human BDNF gene. We studied 
      the effect of different antidepressants on BDNF mRNA expression in human 
      neuroblastoma SH-SY5Y cells. RESULTS: Cultured cells were treated with the 
      antidepressants fluoxetine, reboxetine and desipramine for different time lengths 
      (6, 24, 48 hours). Expression of total BDNF mRNA was analyzed by reverse 
      transcription PCR and levels of different BDNF transcripts were detected by 
      hemi-nested PCR with specific primers. Short-term treatment (6 hours) with 
      reboxetine or desipramine reduced total BDNF, whereas long-term treatment (48 
      hours) significantly increased total BDNF mRNA levels. These changes were 
      accounted for by differential regulation of BDNF IV and VIa/b transcripts. 
      Fluoxetine showed no significant effects. CONCLUSION: This is the first study 
      showing biphasic changes in the expression of total and specific BDNF transcripts 
      in human cells following antidepressant treatments. These findings suggest that 
      biphasic induction of BDNF by antidepressants could be a feature common to 
      rodents and humans and encourage the use of SH-SY5Y cells as a tool for 
      investigation of drug effects on human genes.
FAU - Donnici, Lorena
AU  - Donnici L
AD  - Center of Neuropharmacology, Department of Pharmacological Sciences and Center of 
      Excellence on Neurodegenerative Diseases, University of Milano, Italy. 
      lory80@hotmail.com
FAU - Tiraboschi, Ettore
AU  - Tiraboschi E
FAU - Tardito, Daniela
AU  - Tardito D
FAU - Musazzi, Laura
AU  - Musazzi L
FAU - Racagni, Giorgio
AU  - Racagni G
FAU - Popoli, Maurizio
AU  - Popoli M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080705
PL  - England
TA  - BMC Neurosci
JT  - BMC neuroscience
JID - 100966986
RN  - 0 (Antidepressive Agents)
RN  - 0 (Antidepressive Agents, Second-Generation)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Morpholines)
RN  - 0 (RNA, Messenger)
RN  - 01K63SUP8D (Fluoxetine)
RN  - 947S0YZ36I (Reboxetine)
RN  - TG537D343B (Desipramine)
SB  - IM
MH  - Antidepressive Agents/*pharmacology
MH  - Antidepressive Agents, Second-Generation/pharmacology
MH  - Brain-Derived Neurotrophic Factor/*genetics
MH  - Cell Line, Tumor
MH  - Desipramine/pharmacology
MH  - Fluoxetine/pharmacology
MH  - Gene Expression Regulation, Neoplastic/*drug effects
MH  - Humans
MH  - Morpholines/pharmacology
MH  - Neuroblastoma/genetics/pathology
MH  - RNA, Messenger/genetics/metabolism
MH  - Reboxetine
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Time Factors
PMC - PMC2483719
EDAT- 2008/07/08 09:00
MHDA- 2008/08/30 09:00
PMCR- 2008/07/05
CRDT- 2008/07/08 09:00
PHST- 2008/02/15 00:00 [received]
PHST- 2008/07/05 00:00 [accepted]
PHST- 2008/07/08 09:00 [pubmed]
PHST- 2008/08/30 09:00 [medline]
PHST- 2008/07/08 09:00 [entrez]
PHST- 2008/07/05 00:00 [pmc-release]
AID - 1471-2202-9-61 [pii]
AID - 10.1186/1471-2202-9-61 [doi]
PST - epublish
SO  - BMC Neurosci. 2008 Jul 5;9:61. doi: 10.1186/1471-2202-9-61.