PMID- 18602969
OWN - NLM
STAT- MEDLINE
DCOM- 20081014
LR  - 20211020
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 442
IP  - 1
DP  - 2008 Sep 5
TI  - Low levels of Na, K-ATPase and carbonic anhydrase II during choroid plexus 
      development suggest limited involvement in early CSF secretion.
PG  - 77-80
LID - 10.1016/j.neulet.2008.06.068 [doi]
AB  - In the adult, cerebrospinal fluid (CSF) is produced by the actions of numerous 
      transporters and enzymes creating ion gradients that drive the entry of water 
      into the ventricles via the aquaporin-1 water channels (AQP1). It is not known 
      when in development CSF secretion starts but, in the rat, it has been postulated 
      to occur around the time of birth. However, recent evidence suggests that the 
      secretion may start much earlier, as soon as the lateral choroid plexuses first 
      appear (around E14). PURPOSE: To investigate the developmental profiles of two 
      major enzymes responsible for CSF secretion in the adult, Na, K-ATPase (NKA) and 
      carbonic anhydrase II (CAII). METHODS: The developmental profiles of both enzymes 
      were investigated using immunohistochemistry and Western Blot analysis in tissue 
      from embryonic day (E) 15, 18, postnatal day (P) 0, 9 and adult rats. RESULT: 
      Western Blot analysis showed low levels of NKA at E15 followed by a progressive 
      increase with age. Immunohistochemistry confirmed the presence of NKA on the 
      apical membrane of the lateral ventricular choroid plexus epithelium from E15 
      onwards. Western Blot analysis of CAII was complicated by its presence in blood, 
      but the amount of protein increased with age. Immunohistochemically, CAII 
      appeared in the lateral ventricular choroid plexus between P0 and P9. 
      CONCLUSIONS: The low levels of NKA and CAII during early choroid plexus 
      development indicate that other mechanisms, such as the previously described 
      specific protein transfer across epithelial cells, may be involved in early CSF 
      secretion and movement of water into the cerebral ventricles.
FAU - Johansson, Pia
AU  - Johansson P
AD  - Department of Pharmacology, University of Melbourne, Parkville, Victoria 3010, 
      Australia. piaaj@unimelb.edu.au
FAU - Dziegielewska, Katarzyna
AU  - Dziegielewska K
FAU - Saunders, Norman
AU  - Saunders N
LA  - eng
GR  - R01 NS043949/NS/NINDS NIH HHS/United States
GR  - R01 NS043949-04/NS/NINDS NIH HHS/United States
GR  - R01NS043949-01A1/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20080627
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - EC 4.2.1.- (Carbonic Anhydrase II)
RN  - EC 7.2.2.13 (Sodium-Potassium-Exchanging ATPase)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Carbonic Anhydrase II/*biosynthesis
MH  - Cerebrospinal Fluid/*physiology
MH  - Choroid Plexus/*embryology/*enzymology
MH  - Gene Expression
MH  - Immunohistochemistry
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sodium-Potassium-Exchanging ATPase/*biosynthesis
PMC - PMC2583145
MID - NIHMS74665
EDAT- 2008/07/08 09:00
MHDA- 2008/10/15 09:00
PMCR- 2009/09/05
CRDT- 2008/07/08 09:00
PHST- 2008/05/28 00:00 [received]
PHST- 2008/06/23 00:00 [revised]
PHST- 2008/06/24 00:00 [accepted]
PHST- 2008/07/08 09:00 [pubmed]
PHST- 2008/10/15 09:00 [medline]
PHST- 2008/07/08 09:00 [entrez]
PHST- 2009/09/05 00:00 [pmc-release]
AID - S0304-3940(08)00920-8 [pii]
AID - 10.1016/j.neulet.2008.06.068 [doi]
PST - ppublish
SO  - Neurosci Lett. 2008 Sep 5;442(1):77-80. doi: 10.1016/j.neulet.2008.06.068. Epub 
      2008 Jun 27.