PMID- 18603129
OWN - NLM
STAT- MEDLINE
DCOM- 20080828
LR  - 20080708
IS  - 0076-6879 (Print)
IS  - 0076-6879 (Linking)
VI  - 446
DP  - 2008
TI  - Quantification of vascular endothelial cell apoptosis in vivo.
PG  - 287-92
LID - 10.1016/S0076-6879(08)01617-0 [doi]
AB  - Regulation of vascular endothelial cell survival and apoptosis plays a crucial 
      role during development and numerous physiologic and pathologic processes. 
      Analyzing endothelial apoptosis in vivo is necessary not only for the 
      understanding of many physiologic and pathologic processes but also for 
      evaluating treatments that induce endothelial cell apoptosis. This chapter 
      describes one of the more widely used protocols for detecting and quantifying 
      vascular endothelial cell apoptosis by double-staining vascular endothelial cells 
      with immunofluorescence and terminal deoxynucleotidyl transferase dUTP nick-end 
      labeling (TUNEL).
FAU - Zhang, Xuefeng
AU  - Zhang X
AD  - Department of Surgery, Beth Israel Deaconess Medical Center and Harvard Medical 
      School, Boston, Massachusetts, USA.
FAU - Parangi, Sareh
AU  - Parangi S
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Enzymol
JT  - Methods in enzymology
JID - 0212271
SB  - IM
MH  - Animals
MH  - Apoptosis/*physiology
MH  - Cell Nucleus/ultrastructure
MH  - Endothelium, Vascular/*cytology
MH  - Immunohistochemistry/methods
MH  - In Situ Nick-End Labeling/methods
MH  - Mice
MH  - Microscopy, Fluorescence/methods
MH  - Staining and Labeling/methods
EDAT- 2008/07/08 09:00
MHDA- 2008/08/30 09:00
CRDT- 2008/07/08 09:00
PHST- 2008/07/08 09:00 [pubmed]
PHST- 2008/08/30 09:00 [medline]
PHST- 2008/07/08 09:00 [entrez]
AID - S0076-6879(08)01617-0 [pii]
AID - 10.1016/S0076-6879(08)01617-0 [doi]
PST - ppublish
SO  - Methods Enzymol. 2008;446:287-92. doi: 10.1016/S0076-6879(08)01617-0.