PMID- 18603823
OWN - NLM
STAT- MEDLINE
DCOM- 20080807
LR  - 20190608
IS  - 1880-3873 (Electronic)
IS  - 1340-3478 (Linking)
VI  - 15
IP  - 3
DP  - 2008 Jun
TI  - Up-regulation of cell adhesion molecule genes in human endothelial cells 
      stimulated by lymphotoxin alpha: DNA microarray analysis.
PG  - 160-5
AB  - BACKGROUND: We recently reported that the A252G polymorphism of the 
      Lymphotoxin-alpha (LTA) gene, a member of the tumor necrosis factor family, is 
      strongly related with the onset of acute myocardial infarction; however, the 
      roles of LTA in the development of atherosclerosis remain unclear. METHODS AND 
      RESULTS: Changes in gene expression profile in cultured human umbilical vein 
      (HUVEC) and coronary artery endothelial cells (HCAEC) treated with LTA were 
      analyzed with high density oligonucleotide arrays comprised of 8,500 genes. LTA 
      stimulation at 10 ng/mL for 2 hours profoundly induced gene expression associated 
      with signal transduction, cell adhesion and chemoattraction, such as the nuclear 
      factor of kappa light polypeptide gene enhancer in B-cells (NFkB), endothelial 
      adhesion molecule 1 (E-Selectin), vascular cell adhesion molecule 1 (VCAM1), and 
      monocyte chemotactic protein 1 (MCP1) (2.6, 55.7, 45.3 and 2.8 fold in HUVEC, and 
      2.6, 137.2, 64.0 and 13.0 fold in HCAEC, respectively). Quantitative real-time 
      reverse transcriptase-polymerase chain reaction analysis confirmed that LTA 
      increased the expressions of E-Selectin and VCAM1 in a dose-dependent manner both 
      in HUVEC and HCAEC. CONCLUSION: LTA increased the expression of various genes 
      involved in the process of atherosclerosis or inflammation in human endothelial 
      cells, suggesting the roles of LTA in the development of atherosclerosis.
FAU - Suna, Shinichiro
AU  - Suna S
AD  - Department of Cardiovascular Medicine, Osaka University Graduate School of 
      Medicine, Suita, Japan.
FAU - Sakata, Yasuhiko
AU  - Sakata Y
FAU - Sato, Hiroshi
AU  - Sato H
FAU - Mizuno, Hiroya
AU  - Mizuno H
FAU - Nakatani, Daisaku
AU  - Nakatani D
FAU - Shimizu, Masahiko
AU  - Shimizu M
FAU - Usami, Masaya
AU  - Usami M
FAU - Takashima, Seiji
AU  - Takashima S
FAU - Takeda, Hiroshi
AU  - Takeda H
FAU - Hori, Masatsugu
AU  - Hori M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - J Atheroscler Thromb
JT  - Journal of atherosclerosis and thrombosis
JID - 9506298
RN  - 0 (Lymphotoxin-alpha)
RN  - 0 (Oligonucleotides)
SB  - IM
MH  - Atherosclerosis/metabolism/pathology
MH  - Cell Adhesion
MH  - Cells, Cultured
MH  - Endothelial Cells/*cytology
MH  - Endothelium, Vascular/pathology
MH  - *Gene Expression Regulation
MH  - Humans
MH  - Lymphotoxin-alpha/*pharmacology
MH  - Monocytes/cytology
MH  - Oligonucleotide Array Sequence Analysis/*methods
MH  - Oligonucleotides/chemistry
MH  - *Polymorphism, Genetic
MH  - Signal Transduction
MH  - Umbilical Veins/cytology
MH  - *Up-Regulation
EDAT- 2008/07/08 09:00
MHDA- 2008/08/08 09:00
CRDT- 2008/07/08 09:00
PHST- 2008/07/08 09:00 [pubmed]
PHST- 2008/08/08 09:00 [medline]
PHST- 2008/07/08 09:00 [entrez]
AID - JST.JSTAGE/jat/E553 [pii]
AID - 10.5551/jat.e553 [doi]
PST - ppublish
SO  - J Atheroscler Thromb. 2008 Jun;15(3):160-5. doi: 10.5551/jat.e553.