PMID- 18604514
OWN - NLM
STAT- MEDLINE
DCOM- 20081124
LR  - 20211020
IS  - 0946-2716 (Print)
IS  - 0946-2716 (Linking)
VI  - 86
IP  - 9
DP  - 2008 Sep
TI  - Loss of imprinting of IGF2 and H19, loss of heterozygosity of IGF2R and CTCF, and 
      Helicobacter pylori infection in laryngeal squamous cell carcinoma.
PG  - 1057-66
LID - 10.1007/s00109-008-0369-4 [doi]
AB  - Imprinting analyses of IGF2 and H19, loss of heterozygosity (LOH) analyses of 
      IGF2R and CTCF and Helicobacter pylori detection, were performed on 35 human 
      laryngeal squamous cell carcinomas (LSCC). Forty-six percent of the tumors were 
      heterozygous for IGF2, and 54% were informative for the H19. Biallelic expression 
      of IGF2 was observed in 33% (5 out of 15) of the tumors and in 27% (4 out of 15) 
      of adjacent non-tumorous laryngeal tissues. H19 loss of imprinting (LOI) was 
      observed in 24% (4 out of 17) of the tumors. For IGF2R and CTCF, 71% (25 out of 
      35) and 50% (17/34), respectively, of the samples were heterozygous, and LOH was 
      detected in 12% (3 out of 25) and 6% (1 out of 17), respectively, of the tumors. 
      H. pylori was found in 26% (9/35) of these tumors. Among them, four were 
      informative for the imprinting analysis. The presence of H. pylori had no effect 
      on IGF2/H19 imprinting. Only the H. pylori detection was further broadened with 
      an additional 47 laryngeal tumors, resulting in a total final positivity of close 
      to 16% (13 out of 82). This study represents the largest comprehensive IGF2/H19 
      imprinting study done to date on well-defined samples of human laryngeal 
      carcinomas and corresponding non-tumorous tissue. For the first time, the 
      analyses of IGF2/H19 imprinting have been broadened with LOH analyses of IGF2R 
      and CTCF, with both of these genes acting as modulators of IGF2 and H19 activity. 
      Although there were indications that H. pylori may be present in LSCC, we are the 
      first to show its presence in LSCC by two direct techniques: Giemsa staining and 
      nested-PCR.
FAU - Grbesa, Ivana
AU  - Grbesa I
AD  - Laboratory of Molecular Pathology, Division of Molecular Medicine, Rudjer 
      Boskovic Institute, Zagreb, Croatia. igrbesa@irb.hr
FAU - Marinkovic, Marino
AU  - Marinkovic M
FAU - Ivkic, Mirko
AU  - Ivkic M
FAU - Kruslin, Bozo
AU  - Kruslin B
FAU - Novak-Kujundzic, Renata
AU  - Novak-Kujundzic R
FAU - Pegan, Boris
AU  - Pegan B
FAU - Bogdanovic, Ozren
AU  - Bogdanovic O
FAU - Bedekovic, Vladimir
AU  - Bedekovic V
FAU - Gall-Troselj, Koraljka
AU  - Gall-Troselj K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080705
PL  - Germany
TA  - J Mol Med (Berl)
JT  - Journal of molecular medicine (Berlin, Germany)
JID - 9504370
RN  - 0 (CCCTC-Binding Factor)
RN  - 0 (CTCF protein, human)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (H19 long non-coding RNA)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (RNA, Untranslated)
RN  - 0 (Receptor, IGF Type 2)
RN  - 0 (Repressor Proteins)
RN  - 67763-97-7 (Insulin-Like Growth Factor II)
SB  - IM
MH  - CCCTC-Binding Factor
MH  - *Carcinoma, Squamous Cell/genetics/microbiology/pathology
MH  - DNA-Binding Proteins/*genetics/metabolism
MH  - *Genomic Imprinting
MH  - *Helicobacter Infections
MH  - Helicobacter pylori/metabolism/pathogenicity
MH  - Humans
MH  - Insulin-Like Growth Factor II/*genetics/metabolism
MH  - *Laryngeal Neoplasms/genetics/microbiology/pathology
MH  - Loss of Heterozygosity
MH  - RNA, Long Noncoding
MH  - RNA, Untranslated/*genetics/metabolism
MH  - Receptor, IGF Type 2/*genetics/metabolism
MH  - Repressor Proteins/*genetics/metabolism
EDAT- 2008/07/08 09:00
MHDA- 2008/12/17 09:00
CRDT- 2008/07/08 09:00
PHST- 2006/11/23 00:00 [received]
PHST- 2008/05/09 00:00 [accepted]
PHST- 2008/05/07 00:00 [revised]
PHST- 2008/07/08 09:00 [pubmed]
PHST- 2008/12/17 09:00 [medline]
PHST- 2008/07/08 09:00 [entrez]
AID - 10.1007/s00109-008-0369-4 [doi]
PST - ppublish
SO  - J Mol Med (Berl). 2008 Sep;86(9):1057-66. doi: 10.1007/s00109-008-0369-4. Epub 
      2008 Jul 5.