PMID- 18604639
OWN - NLM
STAT- MEDLINE
DCOM- 20090206
LR  - 20131121
IS  - 1423-0127 (Electronic)
IS  - 1021-7770 (Linking)
VI  - 15
IP  - 6
DP  - 2008 Nov
TI  - Immunohistochemical assessment of cyclic guanosine monophosphate (cGMP) and 
      soluble guanylate cyclase (sGC) within the rostral ventrolateral medulla.
PG  - 801-12
LID - 10.1007/s11373-008-9269-4 [doi]
AB  - Functional evidence suggests that nitric oxide (NO) signalling in the rostral 
      ventrolateral medulla (RVLM) is cGMP-dependent and that this pathway is impaired 
      in hypertension. We examined cGMP expression as a marker of active NO signalling 
      in the C1 region of the RVLM, comparing adult (>18 weeks) Wistar-Kyoto (WKY, n = 
      4) and spontaneously hypertensive rats (SHR, n = 4). Double label 
      immunohistochemistry for cGMP-immunoreactivity (IR) and C1 neurons [as identified 
      by phenylethanolamine N-methyltransferase (PNMT-IR) or tyrosine hydroxylase 
      TH-IR)], or neuronal NO synthase (nNOS) neurones, failed to reveal cGMP-IR 
      neurons in the RVLM of either strain, despite consistent detection of cGMP-IR in 
      the nucleus ambiguus (NA). This was unchanged in the presence of 
      isobutylmethylxanthine (IBMX; 0.5 mM, WKY, n = 4, SHR n = 2) and in young animals 
      (WKY, 10-weeks, n = 3). Incubation of RVLM-slices (WKY, 10-weeks, n = 9) in 
      DETA-NO (100 mum; 10 min) or NMDA (10 muM; 2 min) did not uncover cGMP-IR. In all 
      studies, cGMP was prominent within the vasculature. Soluble guanylate cyclase 
      (sGC)-IR was found throughout neurones of the RVLM, but did not co-localise with 
      PNMT, TH or nNOS-IR neurons (WKY, 10-weeks, n = 6). Results indicate that within 
      the RVLM, cGMP is not detectable using immunohistochemistry in the basal state 
      and cannot be elicited by phosphodiesterase inhibition, NMDA receptor stimulation 
      or NO donor application.
FAU - Powers-Martin, Kellysan
AU  - Powers-Martin K
AD  - Division of Health Sciences, School of Veterinary and Biomedical Science, Murdoch 
      University, South St. Murdoch, Perth, WA, 6150, Australia.
FAU - Barron, Anna M
AU  - Barron AM
FAU - Auckland, Clare H
AU  - Auckland CH
FAU - McCooke, John K
AU  - McCooke JK
FAU - McKitrick, Douglas J
AU  - McKitrick DJ
FAU - Arnolda, Leonard F
AU  - Arnolda LF
FAU - Phillips, Jacqueline K
AU  - Phillips JK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080706
PL  - England
TA  - J Biomed Sci
JT  - Journal of biomedical science
JID - 9421567
RN  - EC 4.6.1.2 (Guanylate Cyclase)
RN  - H2D2X058MU (Cyclic GMP)
SB  - IM
MH  - Animals
MH  - Brain Stem/metabolism
MH  - Cyclic GMP/*metabolism
MH  - Guanylate Cyclase/*metabolism
MH  - Immunohistochemistry
MH  - Male
MH  - Medulla Oblongata/cytology/*metabolism
MH  - Rats
MH  - Rats, Inbred SHR
MH  - Rats, Inbred WKY
EDAT- 2008/07/08 09:00
MHDA- 2009/02/07 09:00
CRDT- 2008/07/08 09:00
PHST- 2007/12/18 00:00 [received]
PHST- 2008/06/29 00:00 [accepted]
PHST- 2008/07/08 09:00 [pubmed]
PHST- 2009/02/07 09:00 [medline]
PHST- 2008/07/08 09:00 [entrez]
AID - 10.1007/s11373-008-9269-4 [doi]
PST - ppublish
SO  - J Biomed Sci. 2008 Nov;15(6):801-12. doi: 10.1007/s11373-008-9269-4. Epub 2008 
      Jul 6.