PMID- 18607895
OWN - NLM
STAT- MEDLINE
DCOM- 20090311
LR  - 20231213
IS  - 1651-2251 (Electronic)
IS  - 0001-6489 (Linking)
VI  - 129
IP  - 1
DP  - 2009 Jan
TI  - Therapeutic influence on circulating and monocyte-derived dendritic cells in 
      laryngeal squamous cell carcinoma patients.
PG  - 84-91
LID - 10.1080/00016480802020459 [doi]
AB  - CONCLUSION: Surgical resection of laryngeal squamous cell carcinoma (LSCC) could 
      be associated with improved circulating myeloid dendritic cell (mDC) number and 
      monocyte-derived dentritic cell (MoDC) function. Although adjunctive radiotherapy 
      after surgery did not effect the normalization of mDC number, it may have an 
      impact on MoDC function. OBJECTIVE: To investigate the effects of surgery and 
      adjunctive radiotherapy on both circulating dendritic cells (DCs) and MoDCs of 
      LSCC patients. SUBJECTS AND METHODS: Forty-six patients with LSCC and 15 
      age-matched healthy control subjects were enrolled in this study. Blood samples 
      were taken before, during, and after conventional treatment from both patients 
      who underwent surgery only (n=18) and those who had adjunctive radiotherapy after 
      tumor removal (n=28). Three-color flow cytometry was used for determination of 
      circulating DC subsets. Moreover, MoDCs were generated utilizing granulocyte 
      macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4), then the 
      phenotype of MoDCs was measured by flow cytometry and the ability to stimulate 
      autologous T cells was tested in a mixed leukocyte reaction (MLR). RESULTS: The 
      preoperative mDC counts, MoDC surface molecular expression and stimulatory 
      capacity were impaired in patients in comparison with controls. The number of 
      mDCs and the expression of CD80, CD83, and HLA-DR on MoDCs were significantly 
      increased as compared with those pretreatment in patients who underwent surgery 
      only and in those who had surgery followed by adjunctive radiotherapy. However, 
      the recovery of CD86 expression and allostimulatory activity was only observed in 
      patients who underwent surgery alone.
FAU - Ma, Xiao Jie
AU  - Ma XJ
AD  - Department of Otolaryngology-Head and Neck Surgery, Qilu Hospital, Wenhua Xi Road 
      107, Jinan, Shandong, China
FAU - Pan, Xin Liang
AU  - Pan XL
FAU - Lv, Zheng Hua
AU  - Lv ZH
FAU - Xu, Feng Lei
AU  - Xu FL
FAU - Liu, Da Yu
AU  - Liu DY
FAU - Lei, Da Peng
AU  - Lei DP
FAU - Xia, Ming
AU  - Xia M
FAU - Luan, Xin Yong
AU  - Luan XY
LA  - eng
PT  - Journal Article
PL  - England
TA  - Acta Otolaryngol
JT  - Acta oto-laryngologica
JID - 0370354
RN  - 0 (Antigens, CD)
RN  - 0 (B7-1 Antigen)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Immunoglobulins)
RN  - 0 (Membrane Glycoproteins)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Antigens, CD/blood
MH  - B7-1 Antigen/blood
MH  - Carcinoma, Squamous Cell/*immunology/pathology/*radiotherapy/*surgery
MH  - Combined Modality Therapy
MH  - Dendritic Cells/*immunology
MH  - Female
MH  - Flow Cytometry
MH  - HLA-DR Antigens/blood
MH  - Humans
MH  - Immune Tolerance/immunology
MH  - Immunoglobulins/blood
MH  - Laryngeal Neoplasms/*immunology/pathology/*radiotherapy/*surgery
MH  - Laryngectomy
MH  - Lymphocyte Activation/immunology
MH  - Lymphocyte Culture Test, Mixed
MH  - Male
MH  - Membrane Glycoproteins/blood
MH  - Middle Aged
MH  - Monocytes/*immunology
MH  - Neck Dissection
MH  - Neoplasm Staging
MH  - Radiotherapy, Adjuvant
MH  - Reference Values
MH  - T-Lymphocytes/immunology
MH  - CD83 Antigen
EDAT- 2008/07/09 09:00
MHDA- 2009/03/12 09:00
CRDT- 2008/07/09 09:00
PHST- 2008/07/09 09:00 [pubmed]
PHST- 2009/03/12 09:00 [medline]
PHST- 2008/07/09 09:00 [entrez]
AID - 794749920 [pii]
AID - 10.1080/00016480802020459 [doi]
PST - ppublish
SO  - Acta Otolaryngol. 2009 Jan;129(1):84-91. doi: 10.1080/00016480802020459.