PMID- 18610739
OWN - NLM
STAT- MEDLINE
DCOM- 20080829
LR  - 20131121
IS  - 0258-851X (Print)
IS  - 0258-851X (Linking)
VI  - 22
IP  - 3
DP  - 2008 May-Jun
TI  - Methylene blue in place of acridine orange as a photosensitizer in photodynamic 
      therapy of osteosarcoma.
PG  - 297-303
AB  - We recently established a unique therapeutic modality for musculoskeletal 
      sarcomas, combining acridine orange (AO) with photodynamic surgery (PDS), 
      photodynamic therapy (PDT) and radiodynamic therapy (RDT); excellent results were 
      obtained in the inhibition of local tumor recurrence after intralesional 
      excision. However, AO is not yet approved for clinical use and intravenous 
      injection. Therefore, methylene blue (MB), which has a very similar chemical 
      structure to AO and is already in clinical use for other diseases, was 
      investigated. In vitro studies using mouse osteosarcoma (LM8) cells revealed that 
      MB-PDT had a strong cytocidal effect and that MB was not radiosensitive, showing 
      no effect in RDT. In vivo studies showed that MB did not specifically accumulate 
      in mouse osteosarcoma tissue and that it did not inhibit tumor growth. MB is not 
      a better photosensitizer than AO in PDS, PDT and RDT for osteosarcoma.
FAU - Matsubara, Takao
AU  - Matsubara T
AD  - Department of Orthopaedic Surgery, Mie Postgraduate School of Medicine, Tsu, 
      Japan.
FAU - Kusuzaki, Katsuyuki
AU  - Kusuzaki K
FAU - Matsumine, Akihiko
AU  - Matsumine A
FAU - Satonaka, Haruhiko
AU  - Satonaka H
FAU - Shintani, Ken
AU  - Shintani K
FAU - Nakamura, Tomoki
AU  - Nakamura T
FAU - Uchida, Atsumasa
AU  - Uchida A
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - In Vivo
JT  - In vivo (Athens, Greece)
JID - 8806809
RN  - 0 (Photosensitizing Agents)
RN  - F30N4O6XVV (Acridine Orange)
RN  - T42P99266K (Methylene Blue)
SB  - IM
MH  - Acridine Orange/chemistry/*therapeutic use
MH  - Animals
MH  - Apoptosis/drug effects/radiation effects
MH  - Cell Line, Tumor
MH  - Male
MH  - Methylene Blue/chemistry/*therapeutic use
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Molecular Structure
MH  - Osteosarcoma/*drug therapy/pathology
MH  - *Photochemotherapy
MH  - Photosensitizing Agents/chemistry/*therapeutic use
MH  - Survival Rate
EDAT- 2008/07/10 09:00
MHDA- 2008/08/30 09:00
CRDT- 2008/07/10 09:00
PHST- 2008/07/10 09:00 [pubmed]
PHST- 2008/08/30 09:00 [medline]
PHST- 2008/07/10 09:00 [entrez]
PST - ppublish
SO  - In Vivo. 2008 May-Jun;22(3):297-303.