PMID- 18614019
OWN - NLM
STAT- MEDLINE
DCOM- 20080725
LR  - 20211020
IS  - 1097-4172 (Electronic)
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 134
IP  - 1
DP  - 2008 Jul 11
TI  - An RNAi screen of chromatin proteins identifies Tip60-p400 as a regulator of 
      embryonic stem cell identity.
PG  - 162-74
LID - 10.1016/j.cell.2008.05.031 [doi]
AB  - Proper regulation of chromatin structure is necessary for the maintenance of cell 
      type-specific gene expression patterns. The embryonic stem cell (ESC) expression 
      pattern governs self-renewal and pluripotency. Here, we present an RNAi screen in 
      mouse ESCs of 1008 loci encoding chromatin proteins. We identified 68 proteins 
      that exhibit diverse phenotypes upon knockdown (KD), including seven subunits of 
      the Tip60-p400 complex. Phenotypic analyses revealed that Tip60-p400 is necessary 
      to maintain characteristic features of ESCs. We show that p400 localization to 
      the promoters of both silent and active genes is dependent upon histone H3 lysine 
      4 trimethylation (H3K4me3). Furthermore, the Tip60-p400 KD gene expression 
      profile is enriched for developmental regulators and significantly overlaps with 
      that of the transcription factor Nanog. Depletion of Nanog reduces p400 binding 
      to target promoters without affecting H3K4me3 levels. Together, these data 
      indicate that Tip60-p400 integrates signals from Nanog and H3K4me3 to regulate 
      gene expression in ESCs.
FAU - Fazzio, Thomas G
AU  - Fazzio TG
AD  - Department of Biochemistry and Biophysics, University of California San 
      Francisco, San Francisco, CA 94158, USA. thomas.fazzio@ucsf.edu
FAU - Huff, Jason T
AU  - Huff JT
FAU - Panning, Barbara
AU  - Panning B
LA  - eng
SI  - GEO/GSE11243
GR  - R01 GM063671/GM/NIGMS NIH HHS/United States
GR  - R01 GM085186/GM/NIGMS NIH HHS/United States
GR  - R01 GM085186-01A1/GM/NIGMS NIH HHS/United States
GR  - R01 GM63671/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (Calcium Channels)
RN  - 0 (Histones)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Inositol 1,4,5-Trisphosphate Receptors)
RN  - 0 (Itpr1 protein, mouse)
RN  - 0 (Nanog Homeobox Protein)
RN  - 0 (Nanog protein, mouse)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Trans-Activators)
RN  - EC 2.3.1.48 (Histone Acetyltransferases)
RN  - EC 2.3.1.48 (Kat5 protein, mouse)
RN  - EC 2.3.1.48 (Lysine Acetyltransferase 5)
SB  - IM
MH  - Animals
MH  - Calcium Channels/*metabolism
MH  - Embryonic Stem Cells/cytology/*metabolism
MH  - Histone Acetyltransferases/*metabolism
MH  - Histones/metabolism
MH  - Homeodomain Proteins/metabolism
MH  - Inositol 1,4,5-Trisphosphate Receptors
MH  - Lysine Acetyltransferase 5
MH  - Mice
MH  - Nanog Homeobox Protein
MH  - Promoter Regions, Genetic
MH  - RNA Interference
MH  - Receptors, Cytoplasmic and Nuclear/*metabolism
MH  - Trans-Activators
MH  - Transcription, Genetic
PMC - PMC4308735
MID - NIHMS190735
EDAT- 2008/07/11 09:00
MHDA- 2008/07/26 09:00
PMCR- 2015/01/28
CRDT- 2008/07/11 09:00
PHST- 2007/11/28 00:00 [received]
PHST- 2008/03/04 00:00 [revised]
PHST- 2008/05/01 00:00 [accepted]
PHST- 2008/07/11 09:00 [pubmed]
PHST- 2008/07/26 09:00 [medline]
PHST- 2008/07/11 09:00 [entrez]
PHST- 2015/01/28 00:00 [pmc-release]
AID - S0092-8674(08)00692-2 [pii]
AID - 10.1016/j.cell.2008.05.031 [doi]
PST - ppublish
SO  - Cell. 2008 Jul 11;134(1):162-74. doi: 10.1016/j.cell.2008.05.031.