PMID- 18615202
OWN - NLM
STAT- MEDLINE
DCOM- 20080924
LR  - 20211020
IS  - 1090-0535 (Electronic)
IS  - 1090-0535 (Linking)
VI  - 14
DP  - 2008 Jul 3
TI  - Microarray analysis of host gene expression for comparison between naive and 
      HSV-1 latent rabbit trigeminal ganglia.
PG  - 1209-21
AB  - PURPOSE: To analyze the rabbit host global gene expression patterns in uninfected 
      and herpes simplex virus type 1 (HSV-1) latent trigeminal ganglia (TG) for 
      identification of host response-initiated transcriptional changes during the 
      maintenance of viral latency. METHODS: The corneas of eight-week-old New Zealand 
      White rabbits were scarified and inoculated with HSV-1 strain McKrae, 5x10(5) 
      plaque forming units/eye. Corneal infection was verified by slit-lamp 
      examination. Prior to sacrifice at 30 days post infection, ocular swabs confirmed 
      no infectious virus was present. TG were aseptically removed from rabbits and 
      placed in RNA stabilization solution. Host RNA was isolated from two groups of 
      TG, uninfected and HSV-1 latent infected, and used to create labeled cRNA. 
      Labeled cRNA was hybridized to two new and novel custom oligonucleotide rabbit 
      arrays, containing a total of 3,123 probes for rabbit genes. RESULTS: The rabbit 
      TG expressed approximately 80% of genes out of a total of 3,123. A one-way ANOVA 
      performed on the log2 transformed signal ratios showed 611 genes were 
      significantly altered (p< or =0.05) in HSV-1 latent TG. These genes, if 
      annotated, were separated by biologic process categories. Five broad categories 
      were most heavily represented: protein processing, carbohydrate processing, cell 
      adhesion, apoptosis, and host defense and immune response. Sixty of the 
      significantly altered genes were found to be altered by more than 2 fold, and 
      five were altered by more than 4 fold. The genes altered by more than 4 fold were 
      all upregulated and related to host defense and immune response. Viral latency 
      had a large effect on protein processing. Of the differentially expressed genes 
      with an assigned biologic process, 90/349 (25.7%) were associated with protein 
      processing. The next most populated categories were carbohydrate metabolism 
      39/349 (11.1%) and host defense and immune response 17/349 (4.9%). CONCLUSIONS: 
      The results of this microarray study demonstrate that host gene expression is 
      altered in the HSV-1 latent rabbit TG. The shift in molecular processes at a 
      pathway level reveals the presence of potential therapeutic significance inherent 
      in the maintenance of HSV-1 latency. This is the first large-scale rabbit gene 
      expression study, using microarray analysis, that documents the involvement of 
      host immunity in maintaining HSV-1 latency.
FAU - Clement, Christian
AU  - Clement C
AD  - Department of Ophthalmology, New Orleans, LA 70112, USA.
FAU - Popp, Michael P
AU  - Popp MP
FAU - Bloom, David C
AU  - Bloom DC
FAU - Schultz, Gregory
AU  - Schultz G
FAU - Liu, Li
AU  - Liu L
FAU - Neumann, Donna M
AU  - Neumann DM
FAU - Bhattacharjee, Partha S
AU  - Bhattacharjee PS
FAU - Hill, James M
AU  - Hill JM
LA  - eng
GR  - R01 EY006311/EY/NEI NIH HHS/United States
GR  - P30 EY002377/EY/NEI NIH HHS/United States
GR  - AI48633/AI/NIAID NIH HHS/United States
GR  - EY006311/EY/NEI NIH HHS/United States
GR  - F32 EY016316/EY/NEI NIH HHS/United States
GR  - EY02377/EY/NEI NIH HHS/United States
GR  - R01 AI048633/AI/NIAID NIH HHS/United States
GR  - F32EY016316/EY/NEI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20080703
PL  - United States
TA  - Mol Vis
JT  - Molecular vision
JID - 9605351
SB  - IM
MH  - Animals
MH  - Down-Regulation/genetics
MH  - *Gene Expression Profiling
MH  - Herpesvirus 1, Human/*physiology
MH  - *Oligonucleotide Array Sequence Analysis
MH  - Rabbits
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Trigeminal Ganglion/*metabolism/*virology
MH  - Up-Regulation
MH  - Virus Latency/*genetics
PMC - PMC2443748
EDAT- 2008/07/11 09:00
MHDA- 2008/09/25 09:00
PMCR- 2008/01/01
CRDT- 2008/07/11 09:00
PHST- 2007/08/13 00:00 [received]
PHST- 2008/04/03 00:00 [accepted]
PHST- 2008/07/11 09:00 [pubmed]
PHST- 2008/09/25 09:00 [medline]
PHST- 2008/07/11 09:00 [entrez]
PHST- 2008/01/01 00:00 [pmc-release]
AID - 142 [pii]
AID - 2007MOLVIS0251 [pii]
PST - epublish
SO  - Mol Vis. 2008 Jul 3;14:1209-21.