PMID- 18616163
OWN - NLM
STAT- MEDLINE
DCOM- 20090908
LR  - 20080711
IS  - 1000-3061 (Print)
IS  - 1000-3061 (Linking)
VI  - 24
IP  - 4
DP  - 2008 Apr
TI  - [RAPD genetic analysis on etiological factor of mink self-biting disease].
PG  - 563-8
AB  - Self-biting is a chronic disease, which cause wound to take effect on mink growth 
      and pelt quality. In this study, we firstly adopted RAPD (random amplification 
      polymorphism DNA) technique based on the reproducible 26 polymorphism primers 
      screened from 100 random primers to analyze hereditary constitution of the 
      samples from healthy minks and self-biting minks, respectively, at molecular 
      level to aim to discuss the causes of self-biting. The results showed that 29 
      straps showed polymorphism among amplified 105 straps, of which the polymorphism 
      rate is 27.62%. Between healthy and sick mink groups, the amplified DNA fragment 
      through different primers indicated different distribution frequency. The 
      similarity coefficient of mink groups is 0.8471 and genetic distance (variation) 
      index is 0.1529. Through primer S356 (whose sequence is CTGCTTAGGG), we amplified 
      different straps between healthy and sick mink. The amplified 1000 bp DNA 
      fragment in the sick mink groups can preliminarily serve as molecular genetic 
      label to distinguish from healthy and sick mink groups to gradually remove the 
      mink individual of self-biting, achieve to purify mink groups and reduce economy 
      loss of mink breeding industry. This work provide theoretical basis for further 
      study on molecular breeding and disease prevention of mink.
FAU - Li, Yumei
AU  - Li Y
AD  - College of Animal Science and Technology, Northeast Agricultural University, 
      Ha'erbin 150030, China.
FAU - Yao, Jiyuan
AU  - Yao J
FAU - Ma, Lina
AU  - Ma L
FAU - Li, Zhiwei
AU  - Li Z
FAU - Bai, Xiujuan
AU  - Bai X
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Sheng Wu Gong Cheng Xue Bao
JT  - Sheng wu gong cheng xue bao = Chinese journal of biotechnology
JID - 9426463
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Genetic Diseases, Inborn/etiology/*genetics/*veterinary
MH  - Mink/*genetics
MH  - Molecular Sequence Data
MH  - *Random Amplified Polymorphic DNA Technique
EDAT- 2008/07/12 09:00
MHDA- 2009/09/09 06:00
CRDT- 2008/07/12 09:00
PHST- 2008/07/12 09:00 [pubmed]
PHST- 2009/09/09 06:00 [medline]
PHST- 2008/07/12 09:00 [entrez]
PST - ppublish
SO  - Sheng Wu Gong Cheng Xue Bao. 2008 Apr;24(4):563-8.