PMID- 18616186
OWN - NLM
STAT- MEDLINE
DCOM- 20090908
LR  - 20080711
IS  - 1000-3061 (Print)
IS  - 1000-3061 (Linking)
VI  - 24
IP  - 4
DP  - 2008 Apr
TI  - [Establishment and evaluation of brain adenosine A2A receptors inactivation model 
      of mice].
PG  - 700-6
AB  - To establish a model of inactivation adenosine A2A receptors in brain tissues of 
      mice, we transplanted bone marrow cells (BMCs) from wild type (WT) C57BL/6 mice 
      into A2A receptor knockout (A2A KO) C57BL/6 mice which were previously 
      fractionated total body irradiation of 6.2 Gyx2. Six weeks later, we identified 
      and evaluated the model. The results showed that the sexual chromagene pattern on 
      white blood cells of recipient mice changed from female pattern to male pattern 
      and there were 95.9% of A2AR+ cells in peripheral white blood cells of recipient 
      mice, whereas there was no significant difference of A2AR mRNA level in brains 
      between these recipient mice and A2AR KO mice. Furthermore, there was no 
      significant difference of breathing frequency, brain water content and level of 
      glutamate between the model mice and WT mice. These results indicated that we 
      established successfully a mouse model of inactivation adenosine A2A receptors in 
      brain tissues. This may provide a new and efficient strategy to study the effect 
      of adenosine A2A receptors in disease and injuries of central nervous system.
FAU - Dai, Shuangshuang
AU  - Dai S
AD  - State Key Laboratory of Trauma, Burn and Combined Injury, Research Institute of 
      Surgery and Daping Hospital, Third Military Medical University, Chongqing 400042, 
      China.
FAU - Wang, Hao
AU  - Wang H
FAU - An, Jianhong
AU  - An J
FAU - Yang, Nan
AU  - Yang N
FAU - Zhou, Yuanguo
AU  - Zhou Y
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Sheng Wu Gong Cheng Xue Bao
JT  - Sheng wu gong cheng xue bao = Chinese journal of biotechnology
JID - 9426463
RN  - 0 (Receptors, Adenosine A2)
SB  - IM
MH  - Animals
MH  - Bone Marrow Transplantation
MH  - Brain/*metabolism
MH  - *Disease Models, Animal
MH  - Female
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Receptors, Adenosine A2/*deficiency/*genetics
MH  - Whole-Body Irradiation
EDAT- 2008/07/12 09:00
MHDA- 2009/09/09 06:00
CRDT- 2008/07/12 09:00
PHST- 2008/07/12 09:00 [pubmed]
PHST- 2009/09/09 06:00 [medline]
PHST- 2008/07/12 09:00 [entrez]
PST - ppublish
SO  - Sheng Wu Gong Cheng Xue Bao. 2008 Apr;24(4):700-6.