PMID- 18616385
OWN - NLM
STAT- MEDLINE
DCOM- 20090602
LR  - 20151119
IS  - 1557-8534 (Electronic)
IS  - 1547-3287 (Linking)
VI  - 18
IP  - 3
DP  - 2009 Apr
TI  - Derivation and characterization of two sibling human embryonic stem cell lines 
      from discarded grade III embryos.
PG  - 423-33
LID - 10.1089/scd.2008.0131 [doi]
AB  - Human embryonic stem (hES) cells are a valuable tool for studying human 
      development in addition to their potential applications in regenerative medicine 
      and drug discovery. The role of genetic background and epigenetic influences in 
      development as well as in response to external influences such as drugs and 
      therapies is well recognized. The great ethnic diversity in the Indian 
      subcontinent translates to interindividual variability in drug response and 
      disease susceptibility. For these reasons, new hES cell lines representing Indian 
      genetic diversity will be valuable in studies of tissue-differentiation, 
      cellular-function and for aspects of characterization of responses to drugs. We 
      have derived two new hES cell lines, BJNhem19 and BJNhem20 from the inner cell 
      mass (ICM) of discarded grade III human embryos that were not suitable for in 
      vitro fertility treatment. Human leukocyte antigen (HLA) isotype analysis shows 
      that they are genetically distinct from existing hES cell lines. Short tandem 
      repeat (STR) analysis shows that the two cell lines are derived from sibling 
      embryos. These cell lines show an undifferentiated phenotype in culture for more 
      than 65 passages, show normal karyotype and express pluripotency markers such as 
      TRA-1-60, TRA-1-81, stage-specific embryonic antigen-4 (SSEA-4), alkaline 
      phosphatase, DNMT3B, GABRB3, GDF3, OCT4, NANOG, SOX2, TERF1, TDGF, LEFTA, THY1, 
      and REX1. While both cell lines can differentiate into derivatives of all three 
      germ layers in vitro, only BJNhem20 can form teratomas when transplanted into 
      mice. We observe an increased frequency of cardiomyocyte differentiation from 
      BJNhem20 embryoid bodies in feeder-free cultures upon induction with DMSO. 
      Cardiomyocytes purified from such cultures survive and show rhythmic contractions 
      for several weeks in culture. These hES cell lines have been accepted for deposit 
      in the U.K. Stem Cell Bank and will be a useful resource for the international 
      stem cell community.
FAU - Inamdar, Maneesha S
AU  - Inamdar MS
AD  - Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore, India. 
      inamdar@jncasr.ac.in
FAU - Venu, Parvathy
AU  - Venu P
FAU - Srinivas, M S
AU  - Srinivas MS
FAU - Rao, Kamini
AU  - Rao K
FAU - VijayRaghavan, K
AU  - VijayRaghavan K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Stem Cells Dev
JT  - Stem cells and development
JID - 101197107
RN  - 0 (Biomarkers)
SB  - IM
MH  - Animals
MH  - Biomarkers/metabolism
MH  - Cell Culture Techniques
MH  - Cell Differentiation
MH  - *Cell Line
MH  - Cell Shape
MH  - Embryo, Mammalian/*cytology
MH  - Embryonic Stem Cells/cytology/*physiology
MH  - Humans
MH  - Karyotyping
MH  - Mice
MH  - Myocytes, Cardiac/*cytology
MH  - Pluripotent Stem Cells/cytology/physiology
EDAT- 2008/07/12 09:00
MHDA- 2009/06/03 09:00
CRDT- 2008/07/12 09:00
PHST- 2008/07/12 09:00 [pubmed]
PHST- 2009/06/03 09:00 [medline]
PHST- 2008/07/12 09:00 [entrez]
AID - 10.1089/scd.2008.0131 [doi]
PST - ppublish
SO  - Stem Cells Dev. 2009 Apr;18(3):423-33. doi: 10.1089/scd.2008.0131.