PMID- 18616586
OWN - NLM
STAT- MEDLINE
DCOM- 20081223
LR  - 20220321
IS  - 0168-6496 (Print)
IS  - 0168-6496 (Linking)
VI  - 66
IP  - 3
DP  - 2008 Dec
TI  - Phylotypes related to Ruminococcus bromii are abundant in the large bowel of 
      humans and increase in response to a diet high in resistant starch.
PG  - 505-15
LID - 10.1111/j.1574-6941.2008.00527.x [doi]
AB  - To further understand how diets containing high levels of fibre protect against 
      colorectal cancer, we examined the effects of diets high in nonstarch 
      polysaccharides (NSP) or high in NSP plus resistant starch (RS) on the 
      composition of the faecal microbial community in 46 healthy adults in a 
      randomized crossover intervention study. Changes in bacterial populations were 
      examined using denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene 
      fragments. Bacterial profiles demonstrated changes in response to the consumption 
      of both RS and NSP diets [analysis of similarities (ANOSIM): R=0.341-0.507, 
      P<0.01]. A number of different DGGE bands with increased intensity in response to 
      dietary intervention were attributed to as-yet uncultivated bacteria closely 
      related to Ruminococcus bromii. A real-time PCR assay specific to the R. bromii 
      group was applied to faecal samples from the dietary study and this group was 
      found to comprise a significant proportion of the total community when 
      individuals consumed their normal diets (4.4+/-2.6% of total 16S rRNA gene 
      abundance) and numbers increased significantly (+/-67%, P<0.05) with the RS, but 
      not the NSP, dietary intervention. This study indicates that R. bromii-related 
      bacteria are abundant in humans and may be significant in the fermentation of 
      complex carbohydrates in the large bowel.
FAU - Abell, Guy C J
AU  - Abell GC
AD  - Preventative Health National Research Flagship, CSIRO Human Nutrition, Adelaide, 
      South Australia, Australia. guy.abell@gmail.com
FAU - Cooke, Caroline M
AU  - Cooke CM
FAU - Bennett, Corinna N
AU  - Bennett CN
FAU - Conlon, Michael A
AU  - Conlon MA
FAU - McOrist, Alexandra L
AU  - McOrist AL
LA  - eng
SI  - GENBANK/EF581095
SI  - GENBANK/EF581096
SI  - GENBANK/EF581097
SI  - GENBANK/EF581098
SI  - GENBANK/EF581099
SI  - GENBANK/EF581100
SI  - GENBANK/EF581101
SI  - GENBANK/EF581102
SI  - GENBANK/EF581103
SI  - GENBANK/EF581104
SI  - GENBANK/EF581105
SI  - GENBANK/EF581106
SI  - GENBANK/EF581107
SI  - GENBANK/EF581108
SI  - GENBANK/EF581109
SI  - GENBANK/EF581110
SI  - GENBANK/EF581111
SI  - GENBANK/EF581112
SI  - GENBANK/EF581113
SI  - GENBANK/EF581114
SI  - GENBANK/EF581115
SI  - GENBANK/EF581116
SI  - GENBANK/EF581117
SI  - GENBANK/EF581118
SI  - GENBANK/EF581119
SI  - GENBANK/EF581120
SI  - GENBANK/EF581121
SI  - GENBANK/EF581122
SI  - GENBANK/EF581123
SI  - GENBANK/EF591651
SI  - GENBANK/EF591652
SI  - GENBANK/EF591653
SI  - GENBANK/EF591654
SI  - GENBANK/EF591655
SI  - GENBANK/EF591656
SI  - GENBANK/EF591657
SI  - GENBANK/EF591658
SI  - GENBANK/EF591659
SI  - GENBANK/EF591660
SI  - GENBANK/EF591661
SI  - GENBANK/EF591662
SI  - GENBANK/EF591663
SI  - GENBANK/EF591664
SI  - GENBANK/EF591665
SI  - GENBANK/EF591666
SI  - GENBANK/EF591667
PT  - Journal Article
PT  - Randomized Controlled Trial
DEP - 20080624
PL  - England
TA  - FEMS Microbiol Ecol
JT  - FEMS microbiology ecology
JID - 8901229
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (Fatty Acids)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 9005-25-8 (Starch)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Bacteria/*classification/*drug effects/genetics/growth & development
MH  - Diet
MH  - Dietary Carbohydrates/*pharmacology
MH  - Fatty Acids/analysis
MH  - Feces/chemistry/microbiology
MH  - Female
MH  - Humans
MH  - Intestine, Large/*microbiology
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Ruminococcus/*classification/*drug effects
MH  - Starch/*pharmacology
EDAT- 2008/07/12 09:00
MHDA- 2008/12/24 09:00
CRDT- 2008/07/12 09:00
PHST- 2008/07/12 09:00 [pubmed]
PHST- 2008/12/24 09:00 [medline]
PHST- 2008/07/12 09:00 [entrez]
AID - FEM527 [pii]
AID - 10.1111/j.1574-6941.2008.00527.x [doi]
PST - ppublish
SO  - FEMS Microbiol Ecol. 2008 Dec;66(3):505-15. doi: 
      10.1111/j.1574-6941.2008.00527.x. Epub 2008 Jun 24.