PMID- 18617057
OWN - NLM
STAT- MEDLINE
DCOM- 20080730
LR  - 20190816
IS  - 1873-4456 (Electronic)
IS  - 0165-4608 (Linking)
VI  - 184
IP  - 2
DP  - 2008 Jul 15
TI  - Analysis of gene rearrangements using a fluorescence in situ hybridization method 
      in Mexican patients with acute lymphoblastic leukemia: experience at a single 
      institution.
PG  - 94-8
LID - 10.1016/j.cancergencyto.2008.04.003 [doi]
AB  - We evaluated the prevalence of BCR/ABL, MLL, and ETV6/RUNX1 rearrangements as 
      well as CDKN2A (alias p16) deletion in a group of Mexican children with acute 
      lymphoblastic leukemia (ALL) to determine whether the changes coexist, and to 
      compare the incidences found with other reports in the literature. To increase 
      the detection of these abnormalities, we combined conventional cytogenetics and 
      fluorescence in situ hybridization (FISH) analysis. Bone marrow samples were 
      obtained from 59 consecutive children with ALL. FISH detected a total of 63 
      abnormalities with the selected probes, 34 of which were related to the 
      conventional cytogenetic results. The most common abnormality was the p16 
      deletion (22.8%), followed by MLL and ETV6/RUNX1 rearrangements (8.7%), and the 
      BCR/ABL fusion was the least frequent (2.7%). The coexistence of two recurrent 
      abnormalities with specific prognostic significance in the same patient was not 
      found. A lesser proportion of the p16 deletion in T-ALL patients was observed, 
      probably related to the low prevalence of this subtype in our population. In 
      addition, we confirmed the low frequency of the ETV6/RUNX1 fusion observed in 
      Hispanics. Due to the different prevalence of these abnormalities in the Mexican 
      population, similar studies should be conducted analyzing new rearrangements, to 
      improve the adequate classification of the abnormalities and the stratification 
      in prognostic groups.
FAU - Perez-Vera, Patricia
AU  - Perez-Vera P
AD  - Department of Research in Human Genetics, Instituto Nacional de Pediatria, 
      Insurgentes Sur 3700-C, Col. Insurgentes Cuicuilco, Mexico City DF 04530, Mexico. 
      pperezvera@yahoo.com
FAU - Salas, Consuelo
AU  - Salas C
FAU - Montero-Ruiz, Oreth
AU  - Montero-Ruiz O
FAU - Frias, Sara
AU  - Frias S
FAU - Dehesa, Gloria
AU  - Dehesa G
FAU - Jarquin, Berenice
AU  - Jarquin B
FAU - Rivera-Luna, Roberto
AU  - Rivera-Luna R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (Core Binding Factor Alpha 2 Subunit)
RN  - 0 (KMT2A protein, human)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (TEL-AML1 fusion protein)
RN  - 149025-06-9 (Myeloid-Lymphoid Leukemia Protein)
RN  - EC 2.1.1.43 (Histone-Lysine N-Methyltransferase)
SB  - IM
MH  - Child
MH  - Child, Preschool
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 1
MH  - Chromosomes, Human, Pair 11
MH  - Chromosomes, Human, Pair 19
MH  - Chromosomes, Human, Pair 22
MH  - Chromosomes, Human, Pair 9
MH  - Core Binding Factor Alpha 2 Subunit/genetics
MH  - Female
MH  - Gene Frequency
MH  - Genes, abl
MH  - Genes, p16
MH  - Histone-Lysine N-Methyltransferase
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Infant
MH  - Infant, Newborn
MH  - Male
MH  - Mexico
MH  - Myeloid-Lymphoid Leukemia Protein/genetics
MH  - Oncogene Proteins, Fusion/genetics
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*genetics
EDAT- 2008/07/12 09:00
MHDA- 2008/07/31 09:00
CRDT- 2008/07/12 09:00
PHST- 2008/02/12 00:00 [received]
PHST- 2008/03/27 00:00 [revised]
PHST- 2008/04/02 00:00 [accepted]
PHST- 2008/07/12 09:00 [pubmed]
PHST- 2008/07/31 09:00 [medline]
PHST- 2008/07/12 09:00 [entrez]
AID - S0165-4608(08)00231-8 [pii]
AID - 10.1016/j.cancergencyto.2008.04.003 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2008 Jul 15;184(2):94-8. doi: 
      10.1016/j.cancergencyto.2008.04.003.