PMID- 18617608
OWN - NLM
STAT- MEDLINE
DCOM- 20090106
LR  - 20211020
IS  - 0013-7227 (Print)
IS  - 1945-7170 (Electronic)
IS  - 0013-7227 (Linking)
VI  - 149
IP  - 11
DP  - 2008 Nov
TI  - Nonclassical mechanisms of progesterone action in the brain: I. Protein kinase C 
      activation in the hypothalamus of female rats.
PG  - 5509-17
LID - 10.1210/en.2008-0712 [doi]
AB  - The modulation of gene regulation by progesterone (P) and its classical 
      intracellular regulation by progestin receptors in the brain, resulting in 
      alterations in physiology and behavior has been well studied. The mechanisms 
      mediating the short latency effects of P are less well understood. Recent studies 
      have revealed rapid nonclassical signaling action of P involving the activation 
      of intracellular signaling pathways. We explored the involvement of protein 
      kinase C (PKC) in P-induced rapid signaling in the ventromedial nucleus of the 
      hypothalamus (VMN) and preoptic area (POA) of the rat brain. Both the 
      Ca2+-independent (basal) PKC activity representing the activation of PKC by the 
      in vivo treatments and the Ca+2-dependent (total) PKC activity assayed in the 
      presence of exogenous cofactors in vitro were determined. A comparison of the two 
      activities demonstrated the strength and temporal status of PKC regulation by 
      steroid hormones in vivo. P treatment resulted in a rapid increase in basal PKC 
      activity in the VMN but not the POA. Estradiol benzoate priming augmented 
      P-initiated increase in PKC basal activity in both the VMN and POA. These 
      increases were inhibited by intracerebroventricular administration of a PKC 
      inhibitor administered 30 min prior to P. The total PKC activity remained 
      unchanged demonstrating maximal PKC activation within 30 min in the VMN. In 
      contrast, P regulation in the POA significantly attenuated total PKC activity +/- 
      estradiol benzoate priming. These rapid changes in P-initiated PKC activity were 
      not due to changes in PKC protein levels or phosphorylation status.
FAU - Balasubramanian, Bhuvana
AU  - Balasubramanian B
AD  - Department of Molecular and Cellular Biology, Baylor College of Medicine, 
      Houston, Texas, USA.
FAU - Portillo, Wendy
AU  - Portillo W
FAU - Reyna, Andrea
AU  - Reyna A
FAU - Chen, Jian Zhong
AU  - Chen JZ
FAU - Moore, Anthony N
AU  - Moore AN
FAU - Dash, Pramod K
AU  - Dash PK
FAU - Mani, Shaila K
AU  - Mani SK
LA  - eng
GR  - R01 NS049160/NS/NINDS NIH HHS/United States
GR  - NS053588/NS/NINDS NIH HHS/United States
GR  - MH072933/MH/NIMH NIH HHS/United States
GR  - R01 NS053588/NS/NINDS NIH HHS/United States
GR  - MH-63954/MH/NIMH NIH HHS/United States
GR  - R01 MH072933/MH/NIMH NIH HHS/United States
GR  - NS049160/NS/NINDS NIH HHS/United States
GR  - R01 MH063954/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20080710
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Indoles)
RN  - 0 (Maleimides)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Type 2)
RN  - L79H6N0V6C (bisindolylmaleimide I)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Brain/*drug effects/physiology
MH  - Calcium/metabolism/pharmacology
MH  - Calcium-Calmodulin-Dependent Protein Kinase Type 2/metabolism
MH  - Cerebral Cortex/drug effects/enzymology/metabolism
MH  - Cyclic AMP-Dependent Protein Kinases/metabolism
MH  - Enzyme Activation/drug effects
MH  - Female
MH  - Hypothalamus/*drug effects/enzymology/metabolism
MH  - Indoles/pharmacology
MH  - Maleimides/pharmacology
MH  - Ovariectomy
MH  - Phosphorylation/drug effects
MH  - Preoptic Area/drug effects/enzymology
MH  - Progesterone/*pharmacology
MH  - Protein Kinase C/antagonists & inhibitors/*metabolism/physiology
MH  - Protein Kinase Inhibitors/pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction/drug effects
PMC - PMC2584599
EDAT- 2008/07/12 09:00
MHDA- 2009/01/07 09:00
PMCR- 2009/11/01
CRDT- 2008/07/12 09:00
PHST- 2008/07/12 09:00 [pubmed]
PHST- 2009/01/07 09:00 [medline]
PHST- 2008/07/12 09:00 [entrez]
PHST- 2009/11/01 00:00 [pmc-release]
AID - en.2008-0712 [pii]
AID - 4348 [pii]
AID - 10.1210/en.2008-0712 [doi]
PST - ppublish
SO  - Endocrinology. 2008 Nov;149(11):5509-17. doi: 10.1210/en.2008-0712. Epub 2008 Jul 
      10.