PMID- 18618144
OWN - NLM
STAT- MEDLINE
DCOM- 20081112
LR  - 20211020
IS  - 0932-0113 (Print)
IS  - 0932-0113 (Linking)
VI  - 103
IP  - 5
DP  - 2008 Oct
TI  - Isolation of Ani s 5, an excretory-secretory and highly heat-resistant allergen 
      useful for the diagnosis of Anisakis larvae sensitization.
PG  - 1231-3
LID - 10.1007/s00436-008-1105-2 [doi]
AB  - Allergens Ani s 1 and Ani s 4 have demonstrated their utility for the diagnosis 
      of the sensitization to larvae of the genus Anisakis. The aim was to determine 
      the number of patients with compatible clinical history, who did not recognize 
      Ani s 1 and Ani s 4, and characterize the allergens responsible for their 
      sensitization. Eighty-four patients were studied by CAP and immunoglobulin E 
      (IgE) immunoblotting. The 12% of the patients recognized allergens different from 
      Ani s 1 and Ani s 4, being half sensitized to a heat-resistant 15-kDa allergen, 
      which was isolated by ethanol fractionation, followed by a hydroxyapatite 
      chromatography and a reversed-phase high-performance liquid chromatography and 
      identified by its amino terminal sequence as Ani s 5. A total of 41 of the 84 
      patients studied (49%) showed specific IgE to Ani s 5 that was detected among the 
      excretory-secretory products and immunohistochemically located at the excretory 
      gland, ventriculus, and the luminal surface of the intestinal epithelium of the 
      larvae.
FAU - Caballero, Maria Luisa
AU  - Caballero ML
AD  - Immunology Department, Hospital Carlos III, Madrid, Spain. mlcsoto@hotmail.com
FAU - Moneo, Ignacio
AU  - Moneo I
FAU - Gomez-Aguado, Fernando
AU  - Gomez-Aguado F
FAU - Corcuera, Maria Teresa
AU  - Corcuera MT
FAU - Casado, Isabel
AU  - Casado I
FAU - Rodriguez-Perez, Rosa
AU  - Rodriguez-Perez R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080710
PL  - Germany
TA  - Parasitol Res
JT  - Parasitology research
JID - 8703571
RN  - 0 (Allergens)
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Helminth Proteins)
SB  - IM
MH  - Allergens/*immunology
MH  - Animals
MH  - Anisakis/*immunology
MH  - Calcium-Binding Proteins/*immunology
MH  - Helminth Proteins/*immunology
MH  - Larva/immunology
EDAT- 2008/07/12 09:00
MHDA- 2008/11/13 09:00
CRDT- 2008/07/12 09:00
PHST- 2008/01/16 00:00 [received]
PHST- 2008/06/19 00:00 [accepted]
PHST- 2008/07/12 09:00 [pubmed]
PHST- 2008/11/13 09:00 [medline]
PHST- 2008/07/12 09:00 [entrez]
AID - 10.1007/s00436-008-1105-2 [doi]
PST - ppublish
SO  - Parasitol Res. 2008 Oct;103(5):1231-3. doi: 10.1007/s00436-008-1105-2. Epub 2008 
      Jul 10.