PMID- 18619637
OWN - NLM
STAT- MEDLINE
DCOM- 20080923
LR  - 20231213
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 378
IP  - 2
DP  - 2008 Sep 1
TI  - Development and pathogenic evaluation of recombinant herpes simplex virus type 1 
      expressing two fluorescent reporter genes from different lytic promoters.
PG  - 254-64
LID - 10.1016/j.virol.2008.05.034 [doi]
AB  - To develop means to explore viral gene expression in ganglia without laborious 
      histological sectioning and staining, we created a two color fluorescent 
      recombinant HSV-1, in which enhanced green fluorescent protein (EGFP) and red 
      fluorescent protein (RFP) are expressed from the glycoprotein B (gB) and 
      glycoprotein C (gC) promoters respectively. We show that this virus retained 
      growth and pathogenic capacity both in vitro and in vivo compared to wild type 
      HSV-1; established latent infections with similar genome copy number in 
      trigeminal ganglia (TG); induced a similar HSV-specific CD8(+) T cell infiltrate; 
      did not induce CD8(+) T cells reactive to EGFP or RFP; and reactivated from 
      latency with normal kinetics in ex vivo TG cultures. Fluorescent EGFP expression 
      in plaques surrounding neurons preceded RFP expression and provided highly 
      sensitive detection of reactivation and different stages of infection in ex vivo 
      TG cultures. Expression of both EGFP and RFP in neurons was readily detectable in 
      whole mounts of TG excised during acute infection and following in vivo sodium 
      butyrate-induced reactivation from latency. This virus constitutes a useful 
      reagent for monitoring lytic viral promoter activity in sensory neurons in vivo 
      and in vitro.
FAU - Ramachandran, Srividya
AU  - Ramachandran S
AD  - Graduate Program in Molecular Microbiology and Virology, School of Medicine, 
      University of Pittsburgh, Pittsburgh, PA 15213, USA.
FAU - Knickelbein, Jared E
AU  - Knickelbein JE
FAU - Ferko, Christina
AU  - Ferko C
FAU - Hendricks, Robert L
AU  - Hendricks RL
FAU - Kinchington, Paul R
AU  - Kinchington PR
LA  - eng
GR  - P30 EY008098-199002/EY/NEI NIH HHS/United States
GR  - R01 EY015291-01/EY/NEI NIH HHS/United States
GR  - R01 EY005945-19/EY/NEI NIH HHS/United States
GR  - EY08098/EY/NEI NIH HHS/United States
GR  - R01 EY005945-20/EY/NEI NIH HHS/United States
GR  - EY05945/EY/NEI NIH HHS/United States
GR  - R01 EY015291-04/EY/NEI NIH HHS/United States
GR  - R01 EY005945-21/EY/NEI NIH HHS/United States
GR  - EY015291/EY/NEI NIH HHS/United States
GR  - R01 EY015291-03/EY/NEI NIH HHS/United States
GR  - R01 EY005945/EY/NEI NIH HHS/United States
GR  - R01 EY015291/EY/NEI NIH HHS/United States
GR  - P30 EY008098-189002/EY/NEI NIH HHS/United States
GR  - P30 EY008098-179002/EY/NEI NIH HHS/United States
GR  - R01 EY005945-22/EY/NEI NIH HHS/United States
GR  - R01 EY015291-02/EY/NEI NIH HHS/United States
GR  - P30 EY008098-209002/EY/NEI NIH HHS/United States
GR  - P30 EY008098/EY/NEI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20080711
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Luminescent Proteins)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (enhanced green fluorescent protein)
RN  - 0 (glycoprotein B, Simplexvirus)
RN  - 0 (glycoprotein gC, herpes simplex virus type 1)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
EIN - Virology. 2013 Jan 20;435(2):504
MH  - Animals
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - Chlorocebus aethiops
MH  - *Genes, Reporter
MH  - Green Fluorescent Proteins/genetics/*metabolism
MH  - Herpesvirus 1, Human/*genetics/growth & development/*pathogenicity
MH  - Luminescent Proteins/genetics/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Molecular Biology/*methods
MH  - Neurons/virology
MH  - Organ Culture Techniques
MH  - *Promoter Regions, Genetic
MH  - Trigeminal Ganglion/virology
MH  - Vero Cells
MH  - Viral Envelope Proteins/genetics
MH  - Viral Plaque Assay
MH  - Virus Activation
MH  - Red Fluorescent Protein
PMC - PMC2613845
MID - NIHMS66936
EDAT- 2008/07/16 09:00
MHDA- 2008/09/24 09:00
PMCR- 2009/09/01
CRDT- 2008/07/16 09:00
PHST- 2008/02/05 00:00 [received]
PHST- 2008/05/16 00:00 [revised]
PHST- 2008/05/24 00:00 [accepted]
PHST- 2008/07/16 09:00 [pubmed]
PHST- 2008/09/24 09:00 [medline]
PHST- 2008/07/16 09:00 [entrez]
PHST- 2009/09/01 00:00 [pmc-release]
AID - S0042-6822(08)00380-2 [pii]
AID - 10.1016/j.virol.2008.05.034 [doi]
PST - ppublish
SO  - Virology. 2008 Sep 1;378(2):254-64. doi: 10.1016/j.virol.2008.05.034. Epub 2008 
      Jul 11.