PMID- 18620924
OWN - NLM
STAT- MEDLINE
DCOM- 20081010
LR  - 20130424
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 623
IP  - 2
DP  - 2008 Aug 15
TI  - Development of an optical surface plasmon resonance biosensor assay for 
      (fluoro)quinolones in egg, fish, and poultry meat.
PG  - 195-203
LID - 10.1016/j.aca.2008.06.009 [doi]
AB  - The aim of this study was to develop an optical biosensor inhibition immunoassay, 
      based on the surface plasmon resonance (SPR) principle, for use as a screening 
      test for 13 (fluoro)quinolones, including flumequine, used as veterinary drugs in 
      food-producing animals. For this, we immobilised various quinolone derivatives on 
      the sensor chip and tested binding of a range of different antibodies (polyclonal 
      and one engineered antibody) in the presence and absence of free 
      (fluoro)quinolones. The main challenge was to detect flumequine in an assay 
      giving good results for the other compounds. One antigen-antibody combination 
      proved satisfactory: polyclonal antibodies raised against a dual immunogen and, 
      on the sensor chip, a fluoroquinolone derivative. It was the first time that this 
      concept of the bi-active antibody was described in the literature. The assay, 
      optimised for detection in three matrices (poultry muscle, fish, and egg), was 
      tested on incurred samples prepared by liquid extraction followed by two washing 
      steps. This rapid, simple method proved adequate for detecting at least 13 
      (fluoro)quinolones at concentrations below established maximum residue levels 
      (MRLs). The reference molecule norfloxacin could be detected in the range of 
      0.1-10 microg kg(-1) in extracts of egg and poultry meat and in the range of 
      0.1-100 microg kg(-1) in extracts of fish. The determined midpoints of these 
      calibration curves were about 1, 1.5 and 3 microg kg(-1) in poultry meat, egg and 
      fish, respectively.
FAU - Huet, A-C
AU  - Huet AC
AD  - CER Groupe, Laboratoire d'Hormonologie, Rue Point du Jour 8, 6900 Marloie, 
      Belgium. ac.huet@cergroupe.be
FAU - Charlier, C
AU  - Charlier C
FAU - Singh, G
AU  - Singh G
FAU - Godefroy, S Benrejeb
AU  - Godefroy SB
FAU - Leivo, J
AU  - Leivo J
FAU - Vehniainen, M
AU  - Vehniainen M
FAU - Nielen, M W F
AU  - Nielen MW
FAU - Weigel, S
AU  - Weigel S
FAU - Delahaut, Ph
AU  - Delahaut P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080614
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
RN  - 0 (Antibodies)
RN  - 0 (Buffers)
RN  - 0 (Fluoroquinolones)
RN  - UVG8VSP2SJ (flumequine)
SB  - IM
MH  - Animals
MH  - Antibodies/immunology/metabolism
MH  - Buffers
MH  - Cattle
MH  - Chromatography, Liquid
MH  - Eggs/analysis
MH  - Fishes
MH  - Fluoroquinolones/*analysis/metabolism
MH  - Food Analysis/*methods
MH  - Food Contamination/*analysis
MH  - Immunoassay
MH  - *Optics and Photonics
MH  - Poultry Products/analysis
MH  - Surface Plasmon Resonance/*instrumentation
MH  - Tandem Mass Spectrometry
EDAT- 2008/07/16 09:00
MHDA- 2008/10/11 09:00
CRDT- 2008/07/16 09:00
PHST- 2008/02/01 00:00 [received]
PHST- 2008/05/27 00:00 [revised]
PHST- 2008/06/03 00:00 [accepted]
PHST- 2008/07/16 09:00 [pubmed]
PHST- 2008/10/11 09:00 [medline]
PHST- 2008/07/16 09:00 [entrez]
AID - S0003-2670(08)01082-9 [pii]
AID - 10.1016/j.aca.2008.06.009 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2008 Aug 15;623(2):195-203. doi: 10.1016/j.aca.2008.06.009. Epub 
      2008 Jun 14.