PMID- 18620928
OWN - NLM
STAT- MEDLINE
DCOM- 20081010
LR  - 20131121
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 623
IP  - 2
DP  - 2008 Aug 15
TI  - Capillary electrophoresis-chemiluminescence determination of norfloxacin and 
      prulifloxacin.
PG  - 231-7
LID - 10.1016/j.aca.2008.06.023 [doi]
AB  - A capillary electrophoresis (CE)-chemiluminescence (CL) method for determining 
      norfloxacin (NFLX) and prulifloxacin (PFLX) was developed based on the enhanced 
      CL intensity of the cerium(IV)-sulfite-fluoroquinolone (FQ) reaction sensitized 
      by terbium(III). The separation was conducted in buffer composed of 20 mM sodium 
      citrate, 4 mM citric acid and 10 mM sodium sulfite at pH 6.1. The CL reagent 
      solution consisted of 2 mM cerium(IV), 4 mM terbium(III) and 1.1 mM hydrochloric 
      acid. NFLX and PFLX were baseline separated within 11 min with detection limits 
      (S/N=3) of 0.057 and 0.084 microg mL(-1), respectively. The maximum intra- and 
      inter-day relative standard deviations (R.S.D.s) of migration time of the 
      analytes were less than 4.0% and 4.2%, respectively. The proposed method was 
      applied to detect NFLX and PFLX in fortified urine sample and the results were 
      comparable to high-performance liquid chromatography (HPLC)-UV method. Moreover, 
      the high selectivity of the CL detection and the high-separation efficiency of CE 
      render the method the potential of quick analyzing fluoroquinolones in real 
      complex matrix.
FAU - Yang, Zhongju
AU  - Yang Z
AD  - College of Chemistry, Beijing Normal University, Beijing 100875, PR China.
FAU - Wang, Xiaoli
AU  - Wang X
FAU - Qin, Weidong
AU  - Qin W
FAU - Zhao, Huichun
AU  - Zhao H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080624
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
RN  - 0 (Buffers)
RN  - 0 (Dioxolanes)
RN  - 0 (Fluoroquinolones)
RN  - 0 (Piperazines)
RN  - 06SSF7P179 (Terbium)
RN  - 30K4522N6T (Cerium)
RN  - J42298IESW (prulifloxacin)
RN  - N0F8P22L1P (Norfloxacin)
SB  - IM
MH  - Buffers
MH  - Cerium/chemistry
MH  - Chromatography, High Pressure Liquid
MH  - Dioxolanes/chemistry/*urine
MH  - Electrophoresis, Capillary/*methods
MH  - Fluoroquinolones/chemistry/*urine
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Luminescent Measurements/*methods
MH  - Norfloxacin/chemistry/*urine
MH  - Piperazines/chemistry/*urine
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Spectrophotometry, Ultraviolet
MH  - Terbium/chemistry
EDAT- 2008/07/16 09:00
MHDA- 2008/10/11 09:00
CRDT- 2008/07/16 09:00
PHST- 2008/04/04 00:00 [received]
PHST- 2008/06/08 00:00 [revised]
PHST- 2008/06/14 00:00 [accepted]
PHST- 2008/07/16 09:00 [pubmed]
PHST- 2008/10/11 09:00 [medline]
PHST- 2008/07/16 09:00 [entrez]
AID - S0003-2670(08)01197-5 [pii]
AID - 10.1016/j.aca.2008.06.023 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2008 Aug 15;623(2):231-7. doi: 10.1016/j.aca.2008.06.023. Epub 
      2008 Jun 24.