PMID- 18621746
OWN - NLM
STAT- MEDLINE
DCOM- 20081014
LR  - 20211020
IS  - 1096-0929 (Electronic)
IS  - 1096-6080 (Print)
IS  - 1096-0929 (Linking)
VI  - 105
IP  - 2
DP  - 2008 Oct
TI  - The orl rat with inherited cryptorchidism has increased susceptibility to the 
      testicular effects of in utero dibutyl phthalate exposure.
PG  - 360-7
LID - 10.1093/toxsci/kfn140 [doi]
AB  - Phenotype results from interactions between genetics and environment, but for 
      most environmental chemical exposures, such interactions are theoretical. The 
      phenotypic response of the testis to in utero dibutyl phthalate (DBP) exposure 
      was compared between two strains of Long-Evans (LE) rats, the orl substrain with 
      inherited cryptorchidism and an outbred (wt) strain. orl and wt LE rats were 
      exposed daily between gestational day (GD) 12 and GD21 to DBP dose levels ranging 
      from 50 to 200 mg/kg by oral gavage and sensitive phthalate testicular end points 
      examined at either GD19, GD21, or postnatal day (PND) 21. At 50 mg/kg DBP, GD19 
      expression of Cyp17a1, Insl3, and Scarb1 was significantly reduced in orl but not 
      wt testis. At GD21, statistically significant differential strain effects (orl 
      more sensitive than wt) were observed for testicular expression of Scarb1 at 50 
      and 200 mg/kg DBP and Star at 200 mg/kg DBP. Similarly, DBP exposure 
      disproportionately increased GD21 seminiferous cord diameters and numbers of 
      multinucleated germ cells in the orl strain. At PND21, body weight-corrected 
      testis weights were lowered significantly by DBP exposure at all dose levels in 
      the orl strain but not in wt rats. While the frequency of undescended testes 
      after 200 mg/kg DBP exposure in the orl strain appeared increased, these data 
      were not statistically significant. These results demonstrated enhanced 
      sensitivity of the orl rat to phthalate exposure as compared to its parent 
      strain, a potentially important model of the effects of gene-environment 
      interaction on development of male reproductive malformations.
FAU - Johnson, Kamin J
AU  - Johnson KJ
AD  - Nemours Biomedical Research, Alfred I. duPont Hospital for Children, Wilmington, 
      Delaware 19803, USA. johnson@medsci.udel.edu
FAU - McCahan, Suzanne M
AU  - McCahan SM
FAU - Si, Xiaoli
AU  - Si X
FAU - Campion, Liam
AU  - Campion L
FAU - Herrmann, Revital
AU  - Herrmann R
FAU - Barthold, Julia S
AU  - Barthold JS
LA  - eng
GR  - P20 RR020173/RR/NCRR NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20080710
PL  - United States
TA  - Toxicol Sci
JT  - Toxicological sciences : an official journal of the Society of Toxicology
JID - 9805461
RN  - 0 (Insulin)
RN  - 0 (Leydig insulin-like protein)
RN  - 0 (Phosphoproteins)
RN  - 0 (Proteins)
RN  - 0 (Scarb1 protein, rat)
RN  - 0 (Scavenger Receptors, Class B)
RN  - 0 (steroidogenic acute regulatory protein)
RN  - 2286E5R2KE (Dibutyl Phthalate)
RN  - EC 1.14.14.19 (Steroid 17-alpha-Hydroxylase)
SB  - IM
MH  - Animals
MH  - Body Weight
MH  - Cryptorchidism/*embryology/enzymology/genetics/pathology
MH  - Dibutyl Phthalate/*toxicity
MH  - Disease Models, Animal
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Genotype
MH  - Gestational Age
MH  - Insulin/metabolism
MH  - Male
MH  - Organ Size
MH  - Phenotype
MH  - Phosphoproteins/metabolism
MH  - Pregnancy
MH  - *Prenatal Exposure Delayed Effects
MH  - Proteins/metabolism
MH  - Rats
MH  - Rats, Long-Evans
MH  - Scavenger Receptors, Class B/metabolism
MH  - Steroid 17-alpha-Hydroxylase/metabolism
MH  - Testis/*drug effects/embryology/enzymology/pathology
PMC - PMC2527641
EDAT- 2008/07/16 09:00
MHDA- 2008/10/15 09:00
PMCR- 2009/10/01
CRDT- 2008/07/16 09:00
PHST- 2008/07/16 09:00 [pubmed]
PHST- 2008/10/15 09:00 [medline]
PHST- 2008/07/16 09:00 [entrez]
PHST- 2009/10/01 00:00 [pmc-release]
AID - kfn140 [pii]
AID - 10.1093/toxsci/kfn140 [doi]
PST - ppublish
SO  - Toxicol Sci. 2008 Oct;105(2):360-7. doi: 10.1093/toxsci/kfn140. Epub 2008 Jul 10.