PMID- 18623133
OWN - NLM
STAT- MEDLINE
DCOM- 20080904
LR  - 20221207
IS  - 1098-2825 (Electronic)
IS  - 0887-8013 (Print)
IS  - 0887-8013 (Linking)
VI  - 22
IP  - 4
DP  - 2008
TI  - Association study of CCR5 delta 32 polymorphism among the HLA-DRB1 Caucasian 
      population in Northern Parana, Brazil.
PG  - 229-33
LID - 10.1002/jcla.20225 [doi]
AB  - Chemokines are important determinants of early inflammatory response. The CC 
      chemokine receptor 5 (CCR5) delta 32 variant results in a nonfunctional form of 
      the chemokine receptor and has been implicated in a variety of immune-mediated 
      diseases. In the present study, polymerase chain reaction (PCR) for genomic 
      deoxyribonucleic acid (DNA) samples, using specific CCR5 oligonucleotide primers 
      surrounding the breakpoint deletion, detected a 225-basepair (bp) product from 
      the normal CCR5 allele and a 193-bp product from the 32 bp deletion allele. Human 
      leukocyte antigen (HLA) class II (DRB1) typing was performed by 
      PCR-sequence-specific primer (PCR-SSP). The aim of this study was to evaluate the 
      association of HLA-DRB1 and CCR5 genetic polymorphisms. To evaluate the frequency 
      distributions of CCR5 delta 32 polymorphisms in a Brazilian population and their 
      association with allelic distribution of HLA genes, DRB1; a total of 120 
      Caucasian individuals from northern Parana, Brazil, were tested. The CCR5/CCR5 
      genotype was found in 108 individuals (90%) and only one carried the CCR5 delta 
      32 allele homozygous genotype (0.0238), while 12 (10%) carried the CCR5 delta 32 
      allele heterozygous genotype. The observed frequency for the CCR5 delta 32 allele 
      was 0.05 in the population studied. The results revealed a CCR5 delta 32 allele 
      occurrence with HLA-DRB1(*)01 and DRB1(*)04 (P<0.05). It is possible that 
      HLA-DRB1(*)01 and DRB1(*)04 alleles could be associated with the delta 32-bp 
      deletion of CCR5.
FAU - Muxel, Sandra Marcia
AU  - Muxel SM
AD  - Department of Pathological Sciences, State University of Londrina, Londrina, PR, 
      Brazil.
FAU - Borelli, Sueli Donizete
AU  - Borelli SD
FAU - Amarante, Marla Karine
AU  - Amarante MK
FAU - Voltarelli, Julio Cesar
AU  - Voltarelli JC
FAU - Aoki, Mateus Nobrega
AU  - Aoki MN
FAU - de Oliveira, Carlos Eduardo Coral
AU  - de Oliveira CE
FAU - Ehara Watanabe, Maria Angelica
AU  - Ehara Watanabe MA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Lab Anal
JT  - Journal of clinical laboratory analysis
JID - 8801384
RN  - 0 (HLA-DR Antigens)
RN  - 0 (HLA-DRB1 Chains)
RN  - 0 (Receptors, CCR5)
SB  - IM
MH  - Alleles
MH  - Brazil/epidemiology
MH  - Gene Frequency
MH  - Genetic Testing
MH  - Genetics, Population
MH  - HLA-DR Antigens/*genetics/immunology
MH  - HLA-DRB1 Chains
MH  - Humans
MH  - Polymerase Chain Reaction
MH  - *Polymorphism, Single-Stranded Conformational
MH  - Receptors, CCR5/*genetics
MH  - White People/*genetics
PMC - PMC6648960
EDAT- 2008/07/16 09:00
MHDA- 2008/09/05 09:00
PMCR- 2008/07/11
CRDT- 2008/07/16 09:00
PHST- 2008/07/16 09:00 [pubmed]
PHST- 2008/09/05 09:00 [medline]
PHST- 2008/07/16 09:00 [entrez]
PHST- 2008/07/11 00:00 [pmc-release]
AID - JCLA20225 [pii]
AID - 10.1002/jcla.20225 [doi]
PST - ppublish
SO  - J Clin Lab Anal. 2008;22(4):229-33. doi: 10.1002/jcla.20225.