PMID- 18623510
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20080714
IS  - 0006-3592 (Print)
IS  - 0006-3592 (Linking)
VI  - 48
IP  - 5
DP  - 1995 Dec 5
TI  - Countercurrent gradient chromatography: A continuous focusing technique.
PG  - 461-75
AB  - The continuous separation of proteins was performed in a countercurrent gradient 
      chromatography (CGC) system. A magnetically stabilized fluidized bed (MSFB) was 
      used to establish true countercurrent contact of a solid resin with a liquid 
      buffer. STable pH gradients were formed in the system in less than 10 min and 
      remained stable throughout the course of the separation experiment (>2 h). The 
      shape of the pH gradient, which ultimately controls the resolution and purity of 
      the separation, can be controlled by making simple adjustments in the 
      interstitial velocities of the liquid and solid phases. We have performed the 
      separation of myoglobin and human serum albumin (HSA) using this device and 
      achieved concentration factors of 1.75 for myoglobin and 1.2 for HSA. A 
      mathematical model that has no adjustable parameters has been developed that 
      predicts the focusing behaviour and capabilities of the CGC system. Using the 
      model, we have estimated the optimum phase velocities, particle diameters, and 
      equilibrium parameters necessary for achieving high purity and high 
      concentrations. (c) 1995 John Wiley & Sons, Inc.
FAU - Evans, L L
AU  - Evans LL
AD  - Department of Chemical Engineering, University of Michigan, Ann Arbor, Michigan 
      48109-2136.
FAU - Burns, M A
AU  - Burns MA
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biotechnol Bioeng
JT  - Biotechnology and bioengineering
JID - 7502021
EDAT- 1995/12/05 00:00
MHDA- 1995/12/05 00:01
CRDT- 1995/12/05 00:00
PHST- 1995/12/05 00:00 [pubmed]
PHST- 1995/12/05 00:01 [medline]
PHST- 1995/12/05 00:00 [entrez]
AID - 10.1002/bit.260480508 [doi]
PST - ppublish
SO  - Biotechnol Bioeng. 1995 Dec 5;48(5):461-75. doi: 10.1002/bit.260480508.