PMID- 18624456
OWN - NLM
STAT- MEDLINE
DCOM- 20080917
LR  - 20171116
IS  - 0743-7463 (Print)
IS  - 0743-7463 (Linking)
VI  - 24
IP  - 16
DP  - 2008 Aug 19
TI  - Inverted microcontact printing on polystyrene-block-poly(tert-butyl acrylate) 
      films: a versatile approach to fabricate structured biointerfaces across the 
      length scales.
PG  - 8841-9
LID - 10.1021/la800770y [doi]
AB  - The combination of the recently introduced soft lithographic technique of 
      inverted microcontact printing (i-muCP) and spin-coated films of polystyrene- 
      block-poly( tert-butyl acrylate) (PS 690- b-P tBA 1210) as a reactive platform is 
      shown to yield a versatile approach for the facile fabrication of topographically 
      structured and chemically patterned biointerfaces with characteristic spacings 
      and distances that cross many orders of magnitude. The shortcomings of 
      conventional muCP in printing of small features with large spacings, due to the 
      collapse of small or high aspect ratio stamp structures, are circumvented in 
      i-muCP by printing reactants using a featureless elastomeric stamp onto a 
      topographically structured reactive polymer film. Prior to molecular transfer, 
      the substrate-supported PS 690- b-P tBA 1210 films were structured by imprint 
      lithography resulting in lateral and vertical feature sizes between >50 
      microm-150 nm and >1.0 microm-18 nm, respectively. Time-of-flight secondary ion 
      mass spectrometry (ToF-SIMS) and water contact angle measurements provided 
      evidence for the absence of surface chemical transformations during the 
      imprinting step. Following the previously established hydrolysis and activation 
      protocol with trifluoroacetic acid and N-hydroxysuccinimide, amino 
      end-functionalized poly(ethylene glycol) (PEG-NH 2), as well as bovine serum 
      albumin and fibronectin as model proteins, were successfully transferred by 
      i-muCP and coupled covalently. As shown, i-muCP yields increased PEG coverages 
      and thus improved performance in suppressing nonspecific adsorption of proteins 
      by exploiting the high local concentrations in the micro- and nanocontacts during 
      molecular transfer. The i-muCP strategy provides access to versatile biointerface 
      platforms patterned across the length scales, as shown for guided cancer cell 
      adhesion, which opens the pathway for systematic cell-surface interaction 
      studies.
FAU - Embrechts, Anika
AU  - Embrechts A
AD  - Department of Materials Science and Technology of Polymers, MESA Institute for 
      Nanotechnology and Faculty of Science and Technology, University of Twente, PO 
      Box 217, AE Enschede, The Netherlands.
FAU - Feng, Chuan Liang
AU  - Feng CL
FAU - Mills, Christopher A
AU  - Mills CA
FAU - Lee, Michael
AU  - Lee M
FAU - Bredebusch, Ilona
AU  - Bredebusch I
FAU - Schnekenburger, Jurgen
AU  - Schnekenburger J
FAU - Domschke, Wolfram
AU  - Domschke W
FAU - Vancso, G Julius
AU  - Vancso GJ
FAU - Schonherr, Holger
AU  - Schonherr H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080715
PL  - United States
TA  - Langmuir
JT  - Langmuir : the ACS journal of surfaces and colloids
JID - 9882736
RN  - 0 (Acrylates)
RN  - 0 (Polystyrenes)
RN  - 0 (poly(styrene-b-tert-butylacrylate))
RN  - 27432CM55Q (Serum Albumin, Bovine)
SB  - IM
MH  - Acrylates/*chemistry
MH  - Animals
MH  - Cattle
MH  - Cell Line
MH  - Microscopy, Atomic Force
MH  - Molecular Structure
MH  - Polystyrenes/*chemistry
MH  - Serum Albumin, Bovine/chemistry
EDAT- 2008/07/16 09:00
MHDA- 2008/09/18 09:00
CRDT- 2008/07/16 09:00
PHST- 2008/07/16 09:00 [pubmed]
PHST- 2008/09/18 09:00 [medline]
PHST- 2008/07/16 09:00 [entrez]
AID - 10.1021/la800770y [doi]
PST - ppublish
SO  - Langmuir. 2008 Aug 19;24(16):8841-9. doi: 10.1021/la800770y. Epub 2008 Jul 15.