PMID- 18625010
OWN - NLM
STAT- MEDLINE
DCOM- 20081003
LR  - 20181201
IS  - 0928-8244 (Print)
IS  - 0928-8244 (Linking)
VI  - 53
IP  - 3
DP  - 2008 Aug
TI  - VirB type IV secretory system does not contribute to Brucella suis' avoidance of 
      human dendritic cell maturation.
PG  - 404-12
LID - 10.1111/j.1574-695X.2008.00441.x [doi]
AB  - Dendritic cells (DCs), which are critical components of adaptive immunity, are 
      highly susceptible to infection with the intracellular bacteria Brucella. 
      Infection with living Brucella prevents infected human DCs from engaging in 
      maturation processes, thus impairing their capacity to present antigens to naive 
      T cells and to secrete IL-12. Recently, we have established that several 
      attenuated mutants of Brucella (rough, omp25, bvrR) are unable to control DCs 
      maturation and thus effectively stimulate naive T cells, which could be the 
      origin of the protective immunity elicited by these mutants in vivo. In this 
      study, we investigate the interactions of a VirB-defective Brucella mutant with 
      human DCs to determine whether its attenuation could be attributed to the 
      induction of an adaptive immune response. We show here that in contrast to 
      previously studied strains and similar to wild-type strains, this virB mutant was 
      unable to trigger significant DC maturation. Together with recently published 
      data describing infection with virB mutants in vivo, these results suggest that 
      Brucella T4SS VirB is not involved in the control of DC maturation and does not 
      interfere with the establishment of a T-helper type 1 adaptive immune response.
FAU - Billard, Elisabeth
AU  - Billard E
AD  - Centre d'etude d'agents Pathogenes et Biotechnologies pour la Sante (CPBS) 
      Universite Montpellier 1, Montpellier, France.
FAU - Dornand, Jacques
AU  - Dornand J
FAU - Gross, Antoine
AU  - Gross A
LA  - eng
PT  - Journal Article
DEP - 20080709
PL  - England
TA  - FEMS Immunol Med Microbiol
JT  - FEMS immunology and medical microbiology
JID - 9315554
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Virulence Factors)
SB  - IM
MH  - Brucella suis/genetics/*immunology
MH  - Cell Proliferation
MH  - Dendritic Cells/*immunology/*microbiology
MH  - Gene Deletion
MH  - Humans
MH  - Membrane Transport Proteins/genetics/*immunology
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Virulence Factors/genetics/*immunology
EDAT- 2008/07/16 09:00
MHDA- 2008/10/04 09:00
CRDT- 2008/07/16 09:00
PHST- 2008/07/16 09:00 [pubmed]
PHST- 2008/10/04 09:00 [medline]
PHST- 2008/07/16 09:00 [entrez]
AID - FIM441 [pii]
AID - 10.1111/j.1574-695X.2008.00441.x [doi]
PST - ppublish
SO  - FEMS Immunol Med Microbiol. 2008 Aug;53(3):404-12. doi: 
      10.1111/j.1574-695X.2008.00441.x. Epub 2008 Jul 9.