PMID- 18625476
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20080811
LR  - 20190714
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 83
IP  - 1
DP  - 1977 Nov
TI  - Gene order of the mouse mammary tumor virus glycoproteins.
PG  - 72-83
AB  - Immunochemical and tryptic peptide mapping techniques were used to show that the 
      mouse mammary tumor virus (MMTV) envelope glycoproteins gp52 and gp36 are 
      distinct components derived from a common glycosylated precursor polypeptide of 
      75,000 daltons (gPr75). Because both gp52 and gp36 are derived from a common 
      precursor polypeptide and therefore have a common initiation site, we have been 
      able to determine their gene order within the viral genome. The gene order was 
      deduced from three different types of experiments. The first approach measured 
      the differential inhibition by NaCl hypertonic shock on initiation of gp52 and 
      gp36 synthesis. The second approach measured the kinetics of appearance of 
      various MMTV proteins following the synchronized reinitiation of polypeptide 
      synthesis resulting from NaCl hypertonic shock. The third approach analyzed 
      polypeptides released from polyribosomes after a series of variable-length short 
      pulses with [3H]amino acids. Our results indicate that the gene order for gPr75 
      is H2N-gp52-gp36-COOH and 5'-gp52-gp36-3' within the MMTV genome.
FAU - Schochetman, G
AU  - Schochetman G
AD  - Viral Oncology Program, National Cancer Institute Frederick Cancer Research 
      Center, Frederick, Maryland 21701, USA.
FAU - Oroszlan, S
AU  - Oroszlan S
FAU - Arthur, L
AU  - Arthur L
FAU - Fine, D
AU  - Fine D
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
EDAT- 1977/11/01 00:00
MHDA- 1977/11/01 00:01
CRDT- 1977/11/01 00:00
PHST- 1977/06/23 00:00 [accepted]
PHST- 1977/11/01 00:00 [pubmed]
PHST- 1977/11/01 00:01 [medline]
PHST- 1977/11/01 00:00 [entrez]
AID - 0042-6822(77)90211-2 [pii]
AID - 10.1016/0042-6822(77)90211-2 [doi]
PST - ppublish
SO  - Virology. 1977 Nov;83(1):72-83. doi: 10.1016/0042-6822(77)90211-2.