PMID- 18625613
OWN - NLM
STAT- MEDLINE
DCOM- 20080909
LR  - 20211020
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Print)
IS  - 0305-1048 (Linking)
VI  - 36
IP  - 14
DP  - 2008 Aug
TI  - RNA secondary structure of the feline immunodeficiency virus 5'UTR and Gag coding 
      region.
PG  - 4653-66
LID - 10.1093/nar/gkn447 [doi]
AB  - The 5' untranslated region (5'UTR) of lentiviral genomic RNA is highly 
      structured, and is the site of multiple RNA-RNA and RNA-protein interactions 
      throughout the viral life cycle. The 5'UTR plays a critical role during 
      transcription, translational regulation, genome dimerization, reverse 
      transcription priming and encapsidation. The 5'UTR structures of human 
      lentiviruses have been extensively studied, yet the respective role and 
      conformation of each domain is still controversial. To gain insight into the 
      structure-function relationship of lentiviral 5'UTRs, we modelled the RNA 
      structure of the feline immunodeficiency virus (FIV), a virus that is 
      evolutionarily distant from the primate viruses. Through combined chemical and 
      enzymatic structure probing and a thorough phylogenetic study, we establish a 
      model for the secondary structure of the 5'UTR and Gag coding region. This work 
      highlights properties common to all lentiviruses, like the primer binding site 
      structure and the presence of a stable stem-loop at the 5' extremity. We find 
      that FIV has also evolved specific features, including a long stem loop 
      overlapping the end of the 5'UTR and the beginning of the coding region. In 
      addition, we observed footprints of Gag protein on each side of the initiation 
      codon, this sheds light on the role of the sequences required for encapsidation.
FAU - James, Laurie
AU  - James L
AD  - CNRS UPR2167 - Centre de Genetique Moleculaire, Avenue de la Terrasse, 91190 Gif 
      sur yvette, France.
FAU - Sargueil, Bruno
AU  - Sargueil B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080714
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Gene Products, gag)
RN  - 0 (RNA, Viral)
RN  - 0 (gag Gene Products, Human Immunodeficiency Virus)
RN  - I38ZP9992A (Magnesium)
SB  - IM
MH  - 5' Untranslated Regions/*chemistry/metabolism
MH  - Base Sequence
MH  - Binding Sites
MH  - Dimerization
MH  - Gene Products, gag/*genetics
MH  - HIV-1/genetics
MH  - HIV-2/genetics
MH  - Immunodeficiency Virus, Feline/*genetics
MH  - Magnesium/chemistry
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Nucleic Acid Conformation
MH  - Protein Footprinting
MH  - RNA, Viral/*chemistry/metabolism
MH  - gag Gene Products, Human Immunodeficiency Virus/metabolism
PMC - PMC2504303
EDAT- 2008/07/16 09:00
MHDA- 2008/09/10 09:00
PMCR- 2008/07/14
CRDT- 2008/07/16 09:00
PHST- 2008/07/16 09:00 [pubmed]
PHST- 2008/09/10 09:00 [medline]
PHST- 2008/07/16 09:00 [entrez]
PHST- 2008/07/14 00:00 [pmc-release]
AID - gkn447 [pii]
AID - 10.1093/nar/gkn447 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 2008 Aug;36(14):4653-66. doi: 10.1093/nar/gkn447. Epub 2008 
      Jul 14.