PMID- 18625638
OWN - NLM
STAT- MEDLINE
DCOM- 20080922
LR  - 20231213
IS  - 0961-2033 (Print)
IS  - 0961-2033 (Linking)
VI  - 17
IP  - 7
DP  - 2008 Jul
TI  - Systemic lupus erythematosus patients have increased number of circulating 
      plasmacytoid dendritic cells, but decreased myeloid dendritic cells with 
      deficient CD83 expression.
PG  - 654-62
LID - 10.1177/0961203308089410 [doi]
AB  - Dendritic cells (DCs) are functionally abnormal in systemic lupus erythematosus 
      (SLE). However, previous studies have involved in-vitro cytokine-induced DCs. In 
      this investigation, directly isolated circulating plasmacytoid DCs (pDCs) and 
      myeloid DCs (mDCs) in SLE were studied. Blood dendritic cell antigen (BDCA)-4 and 
      BDCA-1 magnetic isolation kits were used to isolate blood pDCs and mDCs from 30 
      SLE patients and 36 controls. Their number and surface markers, and their 
      relationship with lupus disease activity were evaluated. The percentage of pDCs 
      per peripheral blood mononuclear cells was higher in SLE (0.33+/-0.14) than in 
      controls (0.16+/-0.09, P<0.01), but that of mDCs was lower in SLE (0.43+/-0.14) 
      than in controls (0.63+/-0.32; P<0.01). In controls, both pDCs and mDCs expressed 
      high levels of MHC-II, however, the expression of CD86, CD83 and CCR7 on pDCs 
      were significantly lower than that on mDCs (all P<0.05). mDCs from patients with 
      SLE, particularly those with active disease, expressed lower CD83 than controls. 
      In health, circulating mDCs may be more efficient than pDCs in stimulating T 
      cells. In SLE, the increased number of circulating pDCs supports a pathogenic 
      role for these cells, and the decreased mDC number and CD83 expression may 
      explain the susceptibility to infections in these patients.
FAU - Jin, Ou
AU  - Jin O
AD  - Division of Rheumatology and Clinical Immunology, Department of Medicine, The 
      University of Hong Kong, Hong Kong, China.
FAU - Kavikondala, Sushma
AU  - Kavikondala S
FAU - Sun, Lingyun
AU  - Sun L
FAU - Fu, Rong
AU  - Fu R
FAU - Mok, Mo-Yin
AU  - Mok MY
FAU - Chan, Albert
AU  - Chan A
FAU - Yeung, Joseph
AU  - Yeung J
FAU - Lau, Chak-Sing
AU  - Lau CS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Lupus
JT  - Lupus
JID - 9204265
RN  - 0 (Antigens, CD)
RN  - 0 (Biomarkers)
RN  - 0 (Immunoglobulins)
RN  - 0 (Membrane Glycoproteins)
SB  - IM
MH  - Adult
MH  - Antigens, CD/*immunology
MH  - Biomarkers/metabolism
MH  - Cell Separation
MH  - Cell Shape
MH  - Dendritic Cells/cytology/*immunology
MH  - Female
MH  - Humans
MH  - Immunoglobulins/*immunology
MH  - Immunomagnetic Separation
MH  - Lupus Erythematosus, Systemic/*blood/*immunology/pathology
MH  - Male
MH  - Membrane Glycoproteins/*immunology
MH  - Middle Aged
MH  - Myeloid Cells/cytology/*immunology
MH  - CD83 Antigen
EDAT- 2008/07/16 09:00
MHDA- 2008/09/23 09:00
CRDT- 2008/07/16 09:00
PHST- 2008/07/16 09:00 [pubmed]
PHST- 2008/09/23 09:00 [medline]
PHST- 2008/07/16 09:00 [entrez]
AID - 17/7/654 [pii]
AID - 10.1177/0961203308089410 [doi]
PST - ppublish
SO  - Lupus. 2008 Jul;17(7):654-62. doi: 10.1177/0961203308089410.