PMID- 18626994
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20080715
IS  - 0006-3592 (Print)
IS  - 0006-3592 (Linking)
VI  - 50
IP  - 4
DP  - 1996 May 20
TI  - Osteoblast migration on poly(alpha-hydroxy esters).
PG  - 443-51
AB  - We investigated the migration of rat calvaria osteoblast populations on 
      poly(alpha-hydroxy ester) films for up to 14 days to determine effects of 
      substrate composition and culture conditions on the migratory characteristics of 
      osteoblasts. Initial osteoblast culture conditions included cell colonies formed 
      by seeding a high (84,000 cells/cm(2)) or low (42,000 cells/cm(2)) density of 
      isolated osteoblasts on the polymer films, and bone tissue cultures formed by 
      plating bone chips directly on the substrates. High density osteoblast colonies 
      cultured and allowed to migrate and proliferate radially on 85:15 
      poly(DL-lactic-co-glycolic acid) (PLGA) films, 75:25 PLGA films, and tissue 
      culture polystyrene controls demonstrated that the copolymer ratio in the polymer 
      films did not affect the rate of increase in substrate surface area (or culture 
      area) covered by the growing cell colony. However, the rate of increase in 
      culture area was dependent on the initial osteoblast seeding density. Initial 
      cell colonies formed with a lower osteoblast seeding density on 75:25 PLGA 
      resulted in a lower rate of increase in culture area, specifically 4.9 +/- 0.3 
      mm(2)/day, versus 14.1 +/- 0.7 mm(2)/day for colonies seeded with a higher 
      density of cells on the same polymer films. The proliferation rate for 
      osteoblasts in the high and low density seeded osteoblast colonies did not 
      differ, whereas the proliferation rate for the osteoblasts arising from the bone 
      chips was lower than either of these isolated cell colonies. Confocal and light 
      microscopy revealed that the osteoblast migration occurred as a monolayer of 
      individual osteoblasts and not a calcified tissue front. These results 
      demonstrated that cell seeding conditions strongly affect the rates of osteoblast 
      migration and proliferation on biodegradable poly(alpha-hydroxy esters). (c) 1996 
      John Wiley & Sons, Inc.
FAU - Ishaug, S L
AU  - Ishaug SL
AD  - Cox Laboratory for Biomedical Engineering, Department of Chemical Engineering and 
      Institute of Biosciences and Bioengineering, Rice University, P.O. Box 1892, 
      Houston, Texas 77251.
FAU - Payne, R G
AU  - Payne RG
FAU - Yaszemski, M J
AU  - Yaszemski MJ
FAU - Aufdemorte, T B
AU  - Aufdemorte TB
FAU - Bizios, R
AU  - Bizios R
FAU - Mikos, A G
AU  - Mikos AG
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biotechnol Bioeng
JT  - Biotechnology and bioengineering
JID - 7502021
EDAT- 1996/05/20 00:00
MHDA- 1996/05/20 00:01
CRDT- 1996/05/20 00:00
PHST- 1996/05/20 00:00 [pubmed]
PHST- 1996/05/20 00:01 [medline]
PHST- 1996/05/20 00:00 [entrez]
AID - 10.1002/(SICI)1097-0290(19960520)50:4<443::AID-BIT12>3.0.CO;2-K [doi]
PST - ppublish
SO  - Biotechnol Bioeng. 1996 May 20;50(4):443-51. doi: 
      10.1002/(SICI)1097-0290(19960520)50:4<443::AID-BIT12>3.0.CO;2-K.