PMID- 18627613
OWN - NLM
STAT- MEDLINE
DCOM- 20080903
LR  - 20211020
IS  - 1471-2172 (Electronic)
IS  - 1471-2172 (Linking)
VI  - 9
DP  - 2008 Jul 15
TI  - Leukotriene B4-loaded microspheres: a new therapeutic strategy to modulate cell 
      activation.
PG  - 36
LID - 10.1186/1471-2172-9-36 [doi]
AB  - BACKGROUND: Leukotriene B4 (LTB4) is a potent inflammatory mediator that also 
      stimulates the immune response. In addition, it promotes polymorphonuclear 
      leukocyte phagocytosis, chemotaxis, chemokinesis and modulates cytokines release. 
      Regarding chemical instability of the leukotriene molecule, in the present study 
      we assessed the immunomodulatory activities conferred by LTB4 released from 
      microspheres (MS). A previous oil-in-water emulsion solvent 
      extraction-evaporation method was chosen to prepare LTB4-loaded MS. RESULTS: In 
      the mice cremasteric microcirculation, intraescrotal injection of 0.1 ml of 
      LTB4-loaded MS provoked significant increases in leukocyte rolling flux, adhesion 
      and emigration besides significant decreases in the leukocyte rolling velocity. 
      LTB4-loaded MS also increase peroxisome proliferator-activated receptor-alpha 
      (PPARalpha) expression by murine peritoneal macrophages and stimulate them to 
      generate nitrite levels. Monocyte chemoattractant protein-1 (MCP-1) and nitric 
      oxide (NO) productions were also increased when human umbilical vein and artery 
      endothelial cells (HUVECs and HUAECs, respectively) were stimulated with 
      LTB4-loaded MS. CONCLUSION: LTB4-loaded MS preserve the biological activity of 
      the encapsulated mediator indicating their use as a new strategy to modulate cell 
      activation, especially in the innate immune response.
FAU - Nicolete, Roberto
AU  - Nicolete R
AD  - Departamento de Analises Clinicas, Toxicologicas e Bromatologicas, Faculdade de 
      Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Av, do Cafe 
      s/n, 14040-903, Ribeirao Preto, Sao Paulo, Brasil. nicolete@fcfrp.usp.br; 
      Cristina Rius
FAU - Rius, Cristina
AU  - Rius C
FAU - Piqueras, Laura
AU  - Piqueras L
FAU - Jose, Peter J
AU  - Jose PJ
FAU - Sorgi, Carlos A
AU  - Sorgi CA
FAU - Soares, Edson G
AU  - Soares EG
FAU - Sanz, Maria J
AU  - Sanz MJ
FAU - Faccioli, Lucia H
AU  - Faccioli LH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080715
PL  - England
TA  - BMC Immunol
JT  - BMC immunology
JID - 100966980
RN  - 0 (Chemokine CCL2)
RN  - 0 (Nitrites)
RN  - 0 (PPAR alpha)
RN  - 1HGW4DR56D (Leukotriene B4)
RN  - 31C4KY9ESH (Nitric Oxide)
SB  - IM
MH  - Animals
MH  - Cell Adhesion
MH  - Cell Movement
MH  - Chemokine CCL2/biosynthesis
MH  - Endothelial Cells/*immunology/metabolism
MH  - Humans
MH  - Leukocyte Rolling
MH  - Leukocytes/*immunology
MH  - Leukotriene B4/administration & dosage/*immunology
MH  - Lung/immunology
MH  - Macrophages, Peritoneal/*immunology/metabolism
MH  - Mice
MH  - Microspheres
MH  - Nitric Oxide/metabolism
MH  - Nitrites
MH  - PPAR alpha/metabolism
PMC - PMC2483258
EDAT- 2008/07/17 09:00
MHDA- 2008/09/04 09:00
PMCR- 2008/07/15
CRDT- 2008/07/17 09:00
PHST- 2008/03/19 00:00 [received]
PHST- 2008/07/15 00:00 [accepted]
PHST- 2008/07/17 09:00 [pubmed]
PHST- 2008/09/04 09:00 [medline]
PHST- 2008/07/17 09:00 [entrez]
PHST- 2008/07/15 00:00 [pmc-release]
AID - 1471-2172-9-36 [pii]
AID - 10.1186/1471-2172-9-36 [doi]
PST - epublish
SO  - BMC Immunol. 2008 Jul 15;9:36. doi: 10.1186/1471-2172-9-36.