PMID- 18629072
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20110714
LR  - 20211020
IS  - 1531-6912 (Print)
IS  - 1532-6268 (Electronic)
IS  - 1531-6912 (Linking)
VI  - 5
IP  - 2
DP  - 2004
TI  - A fragment of 21 ORFs around the direct repeat (DR) region of Mycobacterium 
      tuberculosis is absent from the other sequenced mycobacterial genomes: 
      implications for the evolution of the DR region.
PG  - 116-22
LID - 10.1002/cfg.380 [doi]
AB  - The direct repeat (DR) region is a singular locus of the Mycobacterium 
      tuberculosis complex genome. This region consists of 36 bp repetitive sequences 
      separated by non-repetitive unique spacer sequences. Around this region there are 
      several genes coding for proteins of unknown function. To determine whether the 
      M. smegmatis, M. avium, M. marinum and M. leprae genomes contain sequences and 
      ORFs similar to those of the DR locus of the M. tuberculosis complex, we analysed 
      the corresponding regions in these species. As a first step, some conserved genes 
      that flank the DR genes [Rv2785c (rpsO), Rv2786c (ribF), Rv2790c (ltp1 ), Rv2793c 
      (truB), Rv2800, Rv2825, Rv2828, Rv2831 (echA16 ), Rv2838 (rbfA) and Rv2845 (proS 
      )] were used as markers to locate the corresponding orthologues in M. smegmatis, 
      M. avium, M. marinum and M. leprae in silico. Most of these M. tuberculosis 
      marker genes have highly similar orthologues located in the same order and 
      orientation in the other mycobacteria. In contrast, no orthologues were found for 
      ORFs Rv2801-Rv2824, suggesting that these genes are unique to M. tuberculosis 
      within the genus Mycobacterium.We observed that in M. smegmatis and M. avium, 
      Rv2800 and Rv2825 are adjacent. This observation was experimentally confirmed by 
      PCR. In conclusion, as the DR locus and the ORFs around it are absent in M. 
      smegmatis and M. avium and, as it is possible that these species are older than 
      M. tuberculosis, we postulated that the DR locus was acquired by the M. 
      tuberculosis complex species or by an ancestor bacterium.
FAU - Caimi, Karina
AU  - Caimi K
AD  - Instituto de Biotecnologia, CICVyA/INTA, Los Reseros y Las Cabanas, Castelar 
      1712, Argentina.
FAU - Cataldi, Angel
AU  - Cataldi A
LA  - eng
PT  - Journal Article
PL  - Egypt
TA  - Comp Funct Genomics
JT  - Comparative and functional genomics
JID - 101090797
PMC - PMC2447356
EDAT- 2008/07/17 09:00
MHDA- 2008/07/17 09:01
PMCR- 2004/03/01
CRDT- 2008/07/17 09:00
PHST- 2003/07/24 00:00 [received]
PHST- 2003/12/15 00:00 [revised]
PHST- 2003/12/22 00:00 [accepted]
PHST- 2008/07/17 09:00 [pubmed]
PHST- 2008/07/17 09:01 [medline]
PHST- 2008/07/17 09:00 [entrez]
PHST- 2004/03/01 00:00 [pmc-release]
AID - S1531691204000178 [pii]
AID - 10.1002/cfg.380 [doi]
PST - ppublish
SO  - Comp Funct Genomics. 2004;5(2):116-22. doi: 10.1002/cfg.380.