PMID- 18629488
OWN - NLM
STAT- MEDLINE
DCOM- 20090114
LR  - 20211020
IS  - 0093-7711 (Print)
IS  - 0093-7711 (Linking)
VI  - 60
IP  - 10
DP  - 2008 Oct
TI  - Suppressive effect of Elf-1 on FcepsilonRI alpha-chain expression in primary mast 
      cells.
PG  - 557-63
LID - 10.1007/s00251-008-0318-y [doi]
AB  - The high-affinity receptor for immunoglobulin E (IgE), FcepsilonRI, is 
      specifically expressed in mast cells and basophils and plays a key role in 
      IgE-mediated allergic reactions. The transcription factor Elf-1 has been 
      previously identified to bind to the promoter of the human FcepsilonRI 
      alpha-chain, which is essential for the function and expression of FcepsilonRI. 
      In the present study, Elf-1 siRNA was conducted to evaluate the effects of Elf-1 
      on FcepsilonRI alpha-chain expression in the primary mouse mast cells, bone 
      marrow-derived mast cells (BMMC). Introduction of Elf-1 siRNA effectively reduced 
      expression levels of Elf-1 mRNA and protein in BMMC. Transient reporter assay 
      showed that the knockdown of Elf-1 by siRNA resulted in increased FcepsilonRI 
      alpha-chain promoter activity, while overexpression of Elf-1 suppressed 
      alpha-chain promoter activity in BMMC. Elf-1 siRNA-treated BMMC exhibited marked 
      upregulation of FcepsilonRI alpha-chain transcription, whereas beta-chain mRNA 
      was not affected by Elf-1 siRNA. Chromatin immunoprecipitation assay showed that 
      the amount of transcription factor PU.1, recognizing the cis-element close to the 
      Elf-1-site on the FcepsilonRI alpha-chain promoter, was significantly increased 
      by introduction of Elf-1 siRNA. These results indicate that Elf-1 negatively 
      regulates FcepsilonRI alpha-chain expression by suppressing PU.1-mediated 
      transcription of the alpha-chain in BMMC.
FAU - Wang, Qing-Hui
AU  - Wang QH
AD  - Atopy Research Center, Juntendo University School of Medicine, 2-1-1 Hongo, 
      Bunkyo-ku, Tokyo, 113-8421, Japan.
FAU - Nishiyama, Chiharu
AU  - Nishiyama C
FAU - Nakano, Nobuhiro
AU  - Nakano N
FAU - Shimokawa, Naomi
AU  - Shimokawa N
FAU - Hara, Mutsuko
AU  - Hara M
FAU - Kanada, Shunsuke
AU  - Kanada S
FAU - Ogawa, Hideoki
AU  - Ogawa H
FAU - Okumura, Ko
AU  - Okumura K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080716
PL  - United States
TA  - Immunogenetics
JT  - Immunogenetics
JID - 0420404
RN  - 0 (Ephrin-A2)
RN  - 0 (FCER1A protein, human)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Receptors, IgE)
RN  - 0 (Trans-Activators)
RN  - 0 (proto-oncogene protein Spi-1)
RN  - EC 1.13.12.- (Luciferases)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Bone Marrow/metabolism
MH  - Chromatin Immunoprecipitation
MH  - Ephrin-A2/*antagonists & inhibitors
MH  - *Gene Expression Regulation
MH  - Humans
MH  - Luciferases/metabolism
MH  - Mast Cells/*metabolism
MH  - Mice
MH  - Promoter Regions, Genetic/genetics
MH  - Proto-Oncogene Proteins/genetics/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - RNA, Small Interfering/*pharmacology
MH  - Receptors, IgE/*genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Trans-Activators/genetics/metabolism
MH  - Transcription, Genetic
MH  - Up-Regulation
EDAT- 2008/07/17 09:00
MHDA- 2009/01/15 09:00
CRDT- 2008/07/17 09:00
PHST- 2008/04/21 00:00 [received]
PHST- 2008/06/25 00:00 [accepted]
PHST- 2008/07/17 09:00 [pubmed]
PHST- 2009/01/15 09:00 [medline]
PHST- 2008/07/17 09:00 [entrez]
AID - 10.1007/s00251-008-0318-y [doi]
PST - ppublish
SO  - Immunogenetics. 2008 Oct;60(10):557-63. doi: 10.1007/s00251-008-0318-y. Epub 2008 
      Jul 16.