PMID- 18631718
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20080728
LR  - 20190714
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 106
IP  - 1
DP  - 1980 Oct 15
TI  - The housefly virus contains double-stranded RNA.
PG  - 173-6
AB  - The housefly virus (HFV) was shown to contain double-stranded RNA (dsRNA) when 
      examined by fluorescent staining with acridine orange (AO). The pale green color 
      observed was not affected by digestion with single-stranded specific nuclease. In 
      contrast, denatured wet, but not dried virus samples fluoresced red. Viral RNA, 
      extracted and analyzed by polyacrylamide gel electrophoresis, was resolved into 
      10 segments, which were not affected by predigestion with RNase, confirming the 
      ds nature of the genome. The HFV thus belongs to the family Reoviridae. By 
      comparison with dsRNA segments from reovirus type 3, Dearing strain, the housefly 
      virus genome is estimated to have a molecular weight of 17 to 18 x 10(6) daltons.
FAU - Moussa, A Y
AU  - Moussa AY
AD  - School of Zoology, University of New South Wales, P.O. Box 1, Kensington, N.S.W. 
      2033, Australia.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
EDAT- 1980/10/15 00:00
MHDA- 1980/10/15 00:01
CRDT- 1980/10/15 00:00
PHST- 1980/07/03 00:00 [accepted]
PHST- 1980/10/15 00:00 [pubmed]
PHST- 1980/10/15 00:01 [medline]
PHST- 1980/10/15 00:00 [entrez]
AID - 0042-6822(80)90237-8 [pii]
AID - 10.1016/0042-6822(80)90237-8 [doi]
PST - ppublish
SO  - Virology. 1980 Oct 15;106(1):173-6. doi: 10.1016/0042-6822(80)90237-8.