PMID- 1863265
OWN - NLM
STAT- MEDLINE
DCOM- 19910830
LR  - 20131121
IS  - 0158-5231 (Print)
IS  - 0158-5231 (Linking)
VI  - 23
IP  - 1
DP  - 1991 Jan
TI  - Antigen binding diversity of affinity purified autoantibodies against DNA.
PG  - 111-8
AB  - Naturally occurring autoantibodies against native DNA (nDNA) in SLE sera showed 
      diverse antigen binding characteristics. The antibodies isolated by affinity 
      chromatography using nDNA linked to Sepharose 4B exhibited specificity towards 
      nDNA and showed strong reactivity with DNA-psoralen photoadduct by direct binding 
      assay and competitive ELISA. The anti-DNA antibody belong to both IgG and IgM 
      immunoglobulin classes and their ratio was 5:1. The possible significance of 
      altered conformation of nDNA in the etiology of SLE has been discussed.
FAU - Ali, A
AU  - Ali A
AD  - Department of Biochemistry, Faculty of Medicine, Aligarh Muslim University.
FAU - Hasan, R
AU  - Hasan R
FAU - Ali, R
AU  - Ali R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - Biochem Int
JT  - Biochemistry international
JID - 8100311
RN  - 0 (Antibodies, Antinuclear)
RN  - 0 (DNA, Single-Stranded)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Immunoglobulin M)
RN  - 9007-49-2 (DNA)
RN  - 9012-36-6 (Sepharose)
RN  - KTZ7ZCN2EX (Ficusin)
SB  - IM
MH  - Antibodies, Antinuclear/classification/*immunology/isolation & purification
MH  - Antibody Diversity
MH  - Antibody Specificity
MH  - Autoimmune Diseases/immunology
MH  - Binding, Competitive
MH  - Chromatography, Affinity
MH  - Cross Reactions
MH  - DNA/*immunology
MH  - DNA, Single-Stranded/chemistry
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Ficusin/chemistry
MH  - Humans
MH  - Immunoglobulin G/immunology
MH  - Immunoglobulin M/immunology
MH  - Lupus Erythematosus, Systemic/etiology/immunology
MH  - Nucleic Acid Conformation
MH  - Sepharose
EDAT- 1991/01/01 00:00
MHDA- 1991/01/01 00:01
CRDT- 1991/01/01 00:00
PHST- 1991/01/01 00:00 [pubmed]
PHST- 1991/01/01 00:01 [medline]
PHST- 1991/01/01 00:00 [entrez]
PST - ppublish
SO  - Biochem Int. 1991 Jan;23(1):111-8.