PMID- 18633204
OWN - NLM
STAT- MEDLINE
DCOM- 20080911
LR  - 20191111
IS  - 0015-6426 (Print)
IS  - 0015-6426 (Linking)
VI  - 49
IP  - 3
DP  - 2008 Jun
TI  - Analysis of trace residues of tetracyclines in dark-colored honeys by 
      high-performance liquid chromatography using polymeric cartridge and metal 
      chelate affinity chromatography.
PG  - 196-203
AB  - An efficient clean-up procedure was developed for the trace residue determination 
      of tetracyclines (TCs) in dark-colored honeys. TCs were extracted from samples 
      with McIlvaine buffer (pH 4.0) containing 0.01 mol/L Na(2)EDTA. The extracts were 
      treated with both a polymeric cartridge (GL-Pak PLS-2) and a metal chelate 
      affinity column (MCAC) preloaded with copper(II). TCs were eluted and analyzed by 
      high-performance liquid chromatography (HPLC) using fluorescence detection. The 
      method was evaluated for the determination of oxytetracycline (OTC), tetracycline 
      (TC), and chlortetracycline (CTC) in buckwheat honey, because its color is the 
      darkest. The mean recoveries of OTC, TC and CTC from spiked samples, at three 
      fortification levels, were >70%, and the relative standard deviations (RSDs) were 
      <10%. Limits of quantitation (LOQs) of OTC, TC, and CTC were estimated to be 
      0.015 mg/kg, 0.019 mg/kg, and 0.024 mg/kg, respectively.
FAU - Fujita, Kazuhiro
AU  - Fujita K
AD  - Japan Food Research Laboratories, Osaka, Japan.
FAU - Ito, Hironobu
AU  - Ito H
FAU - Ishihara, Michiyo
AU  - Ishihara M
FAU - Inukai, Sachi
AU  - Inukai S
FAU - Tanaka, Hiroyuki
AU  - Tanaka H
FAU - Taniguchi, Makoto
AU  - Taniguchi M
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Shokuhin Eiseigaku Zasshi
JT  - Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan
JID - 0142214
RN  - 0 (Iron Chelating Agents)
RN  - 0 (Tetracyclines)
SB  - IM
MH  - *Chromatography, Affinity
MH  - Chromatography, High Pressure Liquid/*instrumentation
MH  - Color
MH  - Drug Residues/*analysis
MH  - Honey/*analysis
MH  - Iron Chelating Agents
MH  - Tetracyclines/*analysis
EDAT- 2008/07/18 09:00
MHDA- 2008/09/13 09:00
CRDT- 2008/07/18 09:00
PHST- 2008/07/18 09:00 [pubmed]
PHST- 2008/09/13 09:00 [medline]
PHST- 2008/07/18 09:00 [entrez]
AID - JST.JSTAGE/shokueishi/49.196 [pii]
AID - 10.3358/shokueishi.49.196 [doi]
PST - ppublish
SO  - Shokuhin Eiseigaku Zasshi. 2008 Jun;49(3):196-203. doi: 
      10.3358/shokueishi.49.196.