PMID- 1863471
OWN - NLM
STAT- MEDLINE
DCOM- 19910906
LR  - 20181130
IS  - 0920-8534 (Print)
IS  - 0920-8534 (Linking)
VI  - 3
IP  - 2
DP  - 1991 Apr
TI  - Variations in cytotoxicity and isoenzyme patterns of uncloned and cloned cultures 
      of axenic Entamoeba histolytica.
PG  - 75-81
AB  - Five clones of axenic Entamoeba histolytica (HMI) grown as discrete colonies in 
      semisolid agar medium were adapted in liquid medium and labelled as HMI-C121, 
      HMI-C131, HMI-C143, HMI-C144 and HMI-C145. The clone HMI-C121 was more cytotoxic 
      to the cultured Baby Hamster Kidney (BHK) cells while all other clones were 
      significantly (P less than 0.001) less cytotoxic as compared to the cloned 
      HMI-C121 and uncloned E. histolytica (HMI). The uncloned Indian axenic E. 
      histolytica (KCG:0986:11) as well as E. histolytica (NIH:200) cultures were 
      significantly (P less than 0.001) less cytotoxic to cultured BHK cells. No 
      difference in the electromobility of maleate NADP oxidoreductase (ME) or 
      glucophosphate isomerase (GPI) isoenzyme in the lysates of all the cloned and 
      uncloned cultures of E. histolytica was observed. The clones HMI-C121, HMI-C131, 
      HMI-G143 and HMI-C144 had three bands of hexokinase (HK) while all uncloned 
      cultures and one of clones, HMI-C145 had only two bands. Though cloned and 
      uncloned cultures had a single PGM band, the relative electromobility (rf) of 
      phosphoglucomutase (PGM) for clone HMI-C131, HMI-C143 HMI-C144 was relatively 
      less (rf 0.075) and these were also significantly (P less than 0.001) less 
      cytotoxic to BHK cells as compared to clone HMI-C121. It is felt that axenic E. 
      histolytica culture consists of several populations (clones) and expression of 
      isoenzymes pattern or cytotoxic potentials would depend upon the population which 
      predominantly multiples and outgrows other populations in the culture system.
FAU - Romana, S
AU  - Romana S
AD  - Department of Experimental Medicine, Postgraduate Institute of Medical Education 
      and Research, Chandigarh, India.
FAU - Mehta, S K
AU  - Mehta SK
FAU - Vinayak, V K
AU  - Vinayak VK
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - FEMS Microbiol Immunol
JT  - FEMS microbiology immunology
JID - 8901230
RN  - 0 (Isoenzymes)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Clone Cells
MH  - Cricetinae
MH  - Entamoeba histolytica/enzymology/*physiology
MH  - Fibroblasts
MH  - Isoenzymes/*analysis
MH  - Kidney
MH  - Mesocricetus
MH  - Parasitology/*methods
EDAT- 1991/04/01 00:00
MHDA- 1991/04/01 00:01
CRDT- 1991/04/01 00:00
PHST- 1991/04/01 00:00 [pubmed]
PHST- 1991/04/01 00:01 [medline]
PHST- 1991/04/01 00:00 [entrez]
AID - 10.1111/j.1574-6968.1991.tb04200.x [doi]
PST - ppublish
SO  - FEMS Microbiol Immunol. 1991 Apr;3(2):75-81. doi: 
      10.1111/j.1574-6968.1991.tb04200.x.