PMID- 18635870
OWN - NLM
STAT- MEDLINE
DCOM- 20081128
LR  - 20181201
IS  - 1549-4918 (Electronic)
IS  - 1066-5099 (Linking)
VI  - 26
IP  - 10
DP  - 2008 Oct
TI  - Mesenchymal stem cells alter migratory property of T and dendritic cells to delay 
      the development of murine lethal acute graft-versus-host disease.
PG  - 2531-41
LID - 10.1634/stemcells.2008-0146 [doi]
AB  - Due to the potent immunoregulatory capacity, mesenchymal stem cells (MSCs) have 
      been used in clinical trials to treat acute graft-versus-host disease (aGvHD), 
      although the detailed in vivo mechanisms remain elusive. In a murine lethal aGvHD 
      model, MSCs delayed the development of the disease. Interestingly, we found that 
      MSC infusion increased the number of T lymphocytes in the secondary lymphoid 
      organs (SLOs). Since the expression of CD62L and CCR7 is prerequisite for 
      lymphocyte migration into SLOs, the in vitro experiments revealed that in the 
      presence of MSCs, T lymphocytes (including CD4(+)CD25(+) regulatory T cells) 
      preferred to take the naive-like phenotype (CD62L(+)/CCR7(+)) in mixed lymphocyte 
      reaction and maintained the migratory activity elicited by secondary lymphoid 
      tissue chemokine (SLC). Dendritic cells (DCs) are the initiator of immune 
      response. CCR7 expression is pivotal for their maturation and migration into 
      SLOs. However, CCR7 expression and SLC-driven migratory activity of DCs were 
      remarkably suppressed by MSC coculture. The processes above were realized mainly 
      through secretory mechanism. Consistently, MSC infusion maintained T lymphocytes 
      to take CD62L(+)/CCR7(+) phenotype and decreased the CCR7 expression and 
      proportion of DCs in SLOs of aGvHD mice. In conclusion, the altered migratory 
      properties of T cells and DCs might contribute to the immunosuppressive activity 
      of transplanted MSCs in the setting of aGvHD. Disclosure of potential conflicts 
      of interest is found at the end of this article.
FAU - Li, Hong
AU  - Li H
AD  - Department of Cell Biology, Institute of Basic Medical Sciences, Beijing, China. 
      maoning@nic.bmi.ac.cn
FAU - Guo, ZiKuan
AU  - Guo Z
FAU - Jiang, XiaoXia
AU  - Jiang X
FAU - Zhu, Heng
AU  - Zhu H
FAU - Li, XiuSen
AU  - Li X
FAU - Mao, Ning
AU  - Mao N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080717
PL  - England
TA  - Stem Cells
JT  - Stem cells (Dayton, Ohio)
JID - 9304532
RN  - 0 (Antigens, CD)
RN  - 0 (Ccr7 protein, mouse)
RN  - 0 (Receptors, CCR7)
RN  - 126880-86-2 (L-Selectin)
RN  - EC 3.6.5.2 (cdc42 GTP-Binding Protein)
RN  - EC 3.6.5.2 (rac1 GTP-Binding Protein)
SB  - IM
MH  - Acute Disease
MH  - Animals
MH  - Antigens, CD/metabolism
MH  - *Cell Movement
MH  - Coculture Techniques
MH  - Cytoskeleton/metabolism
MH  - Dendritic Cells/*cytology/immunology
MH  - Down-Regulation
MH  - Female
MH  - Graft vs Host Disease/*immunology/*pathology
MH  - Infusions, Intravenous
MH  - L-Selectin/metabolism
MH  - Lymphocyte Count
MH  - Lymphocyte Culture Test, Mixed
MH  - Lymphoid Tissue/immunology/pathology
MH  - Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/*metabolism
MH  - Mice
MH  - Organ Specificity
MH  - Receptors, CCR7/metabolism
MH  - Spleen/pathology
MH  - Subcellular Fractions/metabolism
MH  - T-Lymphocytes/*cytology/immunology
MH  - cdc42 GTP-Binding Protein/metabolism
MH  - rac1 GTP-Binding Protein/metabolism
EDAT- 2008/07/19 09:00
MHDA- 2008/12/17 09:00
CRDT- 2008/07/19 09:00
PHST- 2008/07/19 09:00 [pubmed]
PHST- 2008/12/17 09:00 [medline]
PHST- 2008/07/19 09:00 [entrez]
AID - 2008-0146 [pii]
AID - 10.1634/stemcells.2008-0146 [doi]
PST - ppublish
SO  - Stem Cells. 2008 Oct;26(10):2531-41. doi: 10.1634/stemcells.2008-0146. Epub 2008 
      Jul 17.