PMID- 18637190
OWN - NLM
STAT- MEDLINE
DCOM- 20090206
LR  - 20211020
IS  - 1478-6362 (Electronic)
IS  - 1478-6354 (Print)
IS  - 1478-6354 (Linking)
VI  - 10
IP  - 4
DP  - 2008
TI  - Brain-derived neurotrophic factor and its receptor in the human and the sand rat 
      intervertebral disc.
PG  - R82
LID - 10.1186/ar2456 [doi]
AB  - INTRODUCTION: Brain-derived neurotrophic factor (BDNF) was first identified in 
      the intervertebral disc (IVD) when its molecular upregulation was observed in 
      sections of nucleus pulposus cultured under conditions of increased osmolarity. 
      BDNF is now known to be involved in a number of biologic functions, including 
      regulation of differentiation/survival of sensory neurons, regulation of 
      nociceptive function and central pain modulation, and modulation of inflammatory 
      pain hypersensitivity. In addition, more recent investigations show that BDNF can 
      induce the recruitment of endothelial cells and the formation of vascular 
      structures. The objectives of the present study were to use immunocytochemistry 
      to determine the distribution of BDNF and its receptor (BDNF-tropomyosine 
      receptor kinase B) in the human IVD, and to test for gene expression of BDNF and 
      its receptor in cultured human annulus fibrosus cells. METHODS: We studied 
      immunohistochemical localization of BDNF and its receptor in the human annulus, 
      quantified the percentage of outer annulus and inner annulus cells and nucleus 
      cells positive for BDNF immunolocalization, and studied the gene expression of 
      BDNF and its receptor using microarray analysis. RESULTS: The percentage (mean 
      +/- standard error of the mean) of cells positive for BDNF localization was 
      significantly greater in the outer annulus (32.3 +/- 2.7%, n = 22) compared with 
      either the inner annulus (8.1 +/- 1.5%, n = 6) or the nucleus (10.4 +/- 2.8%, n = 
      3) (P < 0.0001). BDNF-receptor immunolocalization showed a pattern similar to 
      that of BDNF, but was not quantitatively assessed. BDNF gene expression levels 
      from cultured annulus cells showed a significant positive correlation with 
      increasing levels of IVD degeneration (P = 0.011). CONCLUSION: These findings 
      provide data on the presence of BDNF and its receptor in the human IVD at the 
      translational level, and on the expression of BDNF and its receptor by cultured 
      human annulus cells. Our findings point to the need for further studies to define 
      the role of BDNF in the human IVD and to investigate regulatory events within the 
      disc that control the expression of BDNF and its receptor.
FAU - Gruber, Helen E
AU  - Gruber HE
AD  - Department of Orthopaedic Surgery, Carolinas Medical Center, Charlotte, NC 28232, 
      USA. helen.gruber@carolinashealthcare.org
FAU - Ingram, Jane A
AU  - Ingram JA
FAU - Hoelscher, Gretchen
AU  - Hoelscher G
FAU - Zinchenko, Natalia
AU  - Zinchenko N
FAU - Norton, H James
AU  - Norton HJ
FAU - Hanley, Edward N Jr
AU  - Hanley EN Jr
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080717
PL  - England
TA  - Arthritis Res Ther
JT  - Arthritis research & therapy
JID - 101154438
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism
MH  - Cells, Cultured
MH  - Female
MH  - Gene Expression
MH  - Gerbillinae
MH  - Humans
MH  - Intervertebral Disc/cytology/*metabolism
MH  - Male
MH  - Middle Aged
MH  - Rats
MH  - Receptor, trkB/genetics/*metabolism
MH  - Young Adult
PMC - PMC2575628
EDAT- 2008/07/19 09:00
MHDA- 2009/02/07 09:00
PMCR- 2008/07/17
CRDT- 2008/07/19 09:00
PHST- 2008/04/10 00:00 [received]
PHST- 2008/06/17 00:00 [revised]
PHST- 2008/07/17 00:00 [accepted]
PHST- 2008/07/19 09:00 [pubmed]
PHST- 2009/02/07 09:00 [medline]
PHST- 2008/07/19 09:00 [entrez]
PHST- 2008/07/17 00:00 [pmc-release]
AID - ar2456 [pii]
AID - 10.1186/ar2456 [doi]
PST - ppublish
SO  - Arthritis Res Ther. 2008;10(4):R82. doi: 10.1186/ar2456. Epub 2008 Jul 17.