PMID- 18637202
OWN - NLM
STAT- MEDLINE
DCOM- 20081013
LR  - 20220330
IS  - 1744-8069 (Electronic)
IS  - 1744-8069 (Linking)
VI  - 4
DP  - 2008 Jul 17
TI  - Brain derived neurotrophic factor (BDNF) contributes to the pain hypersensitivity 
      following surgical incision in the rats.
PG  - 27
LID - 10.1186/1744-8069-4-27 [doi]
AB  - BACKGROUND: The pathogenic role of brain derived neurotrophic factor (BDNF) in 
      the incisional pain is poorly understood. The present study explores the role of 
      the BDNF in the incision-induced pain hypersensitivity. METHODS: A longitudinal 
      incision was made in one plantar hind paw of isoflurane-anesthetized rats. Dorsal 
      root ganglias (DRG) and spinal cords were removed at various postoperative times 
      (1-72 h). Expression pattern of BDNF was determined by immunohistochemistry and 
      double-labeling immunofluorescence. Lidocaine-induced blockade of sciatic nerve 
      function was used to determine the importance of afferent nerve activity on BDNF 
      expression in the DRG and spinal cord after incision. BDNF antibody was 
      administered intrathecally (IT) or intraperitoneal (IP) to modulate the spinal 
      BDNF or peripheral BDNF after incision. RESULTS: After hind-paw incision, the 
      BDNF was upregulated in the ipsilateral lumbar DRG and spinal cord whereas 
      thoracic BDNF remained unchanged in response to incision. The upregulated BDNF 
      was mainly expressed in the large-sized neurons in DRG and the neurons and the 
      primary nerve terminals in the spinal cord. Sciatic nerve blockade prevented the 
      increase of BDNF in the DRG and spinal cord. IT injection of BDNF antibody 
      greatly inhibited the mechanical allodynia induced by incision whereas IP 
      administration had only marginal effect. CONCLUSION: The present study showed 
      that incision induced the segmental upregulation of BDNF in the DRG and spinal 
      cord through somatic afferent nerve transmission, and the upregulated BDNF 
      contributed to the pain hypersensitivity induced by surgical incision.
FAU - Li, Chang-Qi
AU  - Li CQ
AD  - Department of Anesthesia, Xiang-Ya Second Hospital, Central South University, 
      Changsha, PR China. lichangqi1969@yahoo.com
FAU - Xu, Jun-Mei
AU  - Xu JM
FAU - Liu, Dan
AU  - Liu D
FAU - Zhang, Jian-Yi
AU  - Zhang JY
FAU - Dai, Ru-Ping
AU  - Dai RP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080717
PL  - United States
TA  - Mol Pain
JT  - Molecular pain
JID - 101242662
RN  - 0 (Brain-Derived Neurotrophic Factor)
SB  - IM
MH  - Animal Structures/*surgery
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Cell Size
MH  - Ganglia, Spinal/metabolism
MH  - Male
MH  - Nerve Block
MH  - Neurons, Afferent/metabolism
MH  - Pain/*metabolism/physiopathology
MH  - Protein Transport
MH  - Rats
MH  - Rats, Wistar
MH  - Sciatic Nerve/metabolism/pathology
MH  - Spinal Cord/metabolism
MH  - Synaptic Transmission
MH  - Up-Regulation
PMC - PMC2492846
EDAT- 2008/07/19 09:00
MHDA- 2008/10/14 09:00
PMCR- 2008/07/17
CRDT- 2008/07/19 09:00
PHST- 2008/02/25 00:00 [received]
PHST- 2008/07/17 00:00 [accepted]
PHST- 2008/07/19 09:00 [pubmed]
PHST- 2008/10/14 09:00 [medline]
PHST- 2008/07/19 09:00 [entrez]
PHST- 2008/07/17 00:00 [pmc-release]
AID - 1744-8069-4-27 [pii]
AID - 10.1186/1744-8069-4-27 [doi]
PST - epublish
SO  - Mol Pain. 2008 Jul 17;4:27. doi: 10.1186/1744-8069-4-27.