PMID- 18637904
OWN - NLM
STAT- MEDLINE
DCOM- 20090113
LR  - 20211020
IS  - 1600-0854 (Electronic)
IS  - 1398-9219 (Print)
IS  - 1398-9219 (Linking)
VI  - 9
IP  - 10
DP  - 2008 Sep
TI  - Human herpesvirus-6 induces MVB formation, and virus egress occurs by an exosomal 
      release pathway.
PG  - 1728-42
LID - 10.1111/j.1600-0854.2008.00796.x [doi]
AB  - The final envelopment of most herpesviruses occurs at Golgi or post-Golgi 
      compartments, such as the trans Golgi network (TGN); however, the final 
      envelopment site of human herpesvirus 6 (HHV-6) is uncertain. In this study, we 
      found novel pathways for HHV-6 assembly and release from T cells that differed, 
      in part, from those of alphaherpesviruses. Electron microscopy showed that late 
      in infection, HHV-6-infected cells were larger than uninfected cells and 
      contained many newly formed multivesicular body (MVB)-like compartments that 
      included small vesicles. These MVBs surrounded the Golgi apparatus. Mature 
      virions were found in the MVBs and MVB fusion with plasma membrane, and the 
      release of mature virions together with small vesicles was observed at the cell 
      surface. Immunoelectron microscopy demonstrated that the MVBs contained CD63, an 
      MVB/late endosome marker, and HHV-6 envelope glycoproteins. The viral 
      glycoproteins also localized to internal vesicles in the MVBs and to secreted 
      vesicles (exosomes). Furthermore, we found virus budding at TGN-associated 
      membranes, which expressed CD63, adaptor protein (AP-1) and TGN46, and CD63 
      incorporation into virions. Our findings suggest that mature HHV-6 virions are 
      released together with internal vesicles through MVBs by the cellular exosomal 
      pathway. This scenario has significant implications for understanding HHV-6's 
      maturation pathway.
FAU - Mori, Yasuko
AU  - Mori Y
AD  - Department of Biomedical Research, Laboratory of Virology and Vaccinology, 
      National Institute of Biomedical Innovation, Ibaraki, Osaka, Japan. 
      ymori@nibio.go.jp
FAU - Koike, Masato
AU  - Koike M
FAU - Moriishi, Eiko
AU  - Moriishi E
FAU - Kawabata, Akiko
AU  - Kawabata A
FAU - Tang, Huamin
AU  - Tang H
FAU - Oyaizu, Hiroko
AU  - Oyaizu H
FAU - Uchiyama, Yasuo
AU  - Uchiyama Y
FAU - Yamanishi, Koichi
AU  - Yamanishi K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080711
PL  - England
TA  - Traffic
JT  - Traffic (Copenhagen, Denmark)
JID - 100939340
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, CD)
RN  - 0 (CD63 protein, human)
RN  - 0 (Platelet Membrane Glycoproteins)
RN  - 0 (Tetraspanin 30)
RN  - 0 (Viral Envelope Proteins)
SB  - IM
MH  - Antibodies, Monoclonal
MH  - Antigens, CD/metabolism
MH  - Cell Line
MH  - Cell Membrane/metabolism/ultrastructure/virology
MH  - Endosomes/metabolism/ultrastructure/*virology
MH  - Exosomes/metabolism/ultrastructure/virology
MH  - Golgi Apparatus/metabolism/ultrastructure/virology
MH  - Herpesvirus 6, Human/immunology/metabolism/*physiology/ultrastructure
MH  - Humans
MH  - Immunoprecipitation
MH  - Microscopy, Immunoelectron
MH  - Platelet Membrane Glycoproteins/metabolism
MH  - Tetraspanin 30
MH  - Viral Envelope Proteins/metabolism
MH  - Virion/metabolism/physiology/ultrastructure
MH  - Virus Assembly/*physiology
PMC - PMC2613231
EDAT- 2008/07/22 09:00
MHDA- 2009/01/14 09:00
PMCR- 2009/01/02
CRDT- 2008/07/22 09:00
PHST- 2008/07/22 09:00 [pubmed]
PHST- 2009/01/14 09:00 [medline]
PHST- 2008/07/22 09:00 [entrez]
PHST- 2009/01/02 00:00 [pmc-release]
AID - TRA796 [pii]
AID - 10.1111/j.1600-0854.2008.00796.x [doi]
PST - ppublish
SO  - Traffic. 2008 Sep;9(10):1728-42. doi: 10.1111/j.1600-0854.2008.00796.x. Epub 2008 
      Jul 11.