PMID- 18638562
OWN - NLM
STAT- MEDLINE
DCOM- 20081110
LR  - 20161124
IS  - 1065-6995 (Print)
IS  - 1065-6995 (Linking)
VI  - 32
IP  - 9
DP  - 2008 Sep
TI  - Combination of bone tissue engineering and BMP-2 gene transfection promotes bone 
      healing in osteoporotic rats.
PG  - 1150-7
LID - 10.1016/j.cellbi.2008.06.005 [doi]
AB  - OBJECTIVE: The aim of this study was to develop a feasible approach to promote 
      bone healing in osteoporotic rats using autogenous bone tissue-engineering and 
      gene transfection of human bone morphogenetic protein 2 (hBMP-2). METHODS: Bone 
      marrow stromal cells (BMSCs) from the left tibia of osteoporotic rats were 
      transfected with the hBMP-2 gene in vitro which was confirmed by 
      immunohistochemistry, in situ hybridization and Western blotting. Autogenous 
      transfected or untransfected BMSCs were seeded on macroporous coral 
      hydroxyapatite (CHA) scaffolds. Each cell-scaffold construct was implanted into a 
      defect site which was created in the ramus of the mandible of osteoporotic rats. 
      Four or eight weeks after implantation in situ hybridization was performed in 
      BMSCs transfected with hBMP-2, X-ray examinations, histological and 
      histomorphological analyses were used to evaluate the effect of tissue-engineered 
      bone on osseous defect repair. RESULTS: Newly formed bone was observed at the 
      margin of the defect 4 weeks after implantation with BMSCs transfected with 
      BMP-2. Mature bone was observed 8 weeks after treatment. In the control group 
      there was considerably less new bone and some adipose tissue was observed at the 
      defect margins 8 weeks after implantation. CONCLUSIONS: Autogenous cells 
      transfected with hBMP-2 promote bone formation in osteoporotic rats. 
      BMSC-mediated BMP-2 gene therapy used in conjunction with bone tissue engineering 
      may be used to successfully treat bone defects in osteoporotic rats. This method 
      provides a powerful tool for bone regeneration and other tissue engineering.
FAU - Tang, Youchao
AU  - Tang Y
AD  - Department of Oral & Maxillofacial Surgery, Huizhou Hospital of Stomatology, 
      Guangdong 516001, PR China.
FAU - Tang, Wei
AU  - Tang W
FAU - Lin, Yunfeng
AU  - Lin Y
FAU - Long, Jie
AU  - Long J
FAU - Wang, Hang
AU  - Wang H
FAU - Liu, Lei
AU  - Liu L
FAU - Tian, Weidong
AU  - Tian W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080628
PL  - England
TA  - Cell Biol Int
JT  - Cell biology international
JID - 9307129
RN  - 0 (BMP2 protein, human)
RN  - 0 (Bmp2 protein, rat)
RN  - 0 (Bone Morphogenetic Protein 2)
RN  - 0 (Bone Morphogenetic Proteins)
RN  - 0 (Transforming Growth Factor beta)
RN  - 91D9GV0Z28 (Durapatite)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/metabolism
MH  - Animals
MH  - Bone Marrow Cells/metabolism
MH  - Bone Morphogenetic Protein 2
MH  - Bone Morphogenetic Proteins/*genetics/metabolism/*therapeutic use
MH  - *Bone Regeneration
MH  - Bone and Bones/diagnostic imaging/pathology
MH  - Combined Modality Therapy
MH  - Durapatite
MH  - Female
MH  - Gene Expression Regulation
MH  - *Genetic Therapy
MH  - Humans
MH  - Implants, Experimental
MH  - Osteogenesis
MH  - Osteoporosis/genetics/*therapy
MH  - Ovariectomy
MH  - Radiography
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Stromal Cells/metabolism
MH  - *Tissue Engineering
MH  - *Transfection
MH  - Transforming Growth Factor beta/*genetics/metabolism/*therapeutic use
EDAT- 2008/07/22 09:00
MHDA- 2008/11/11 09:00
CRDT- 2008/07/22 09:00
PHST- 2007/11/20 00:00 [received]
PHST- 2008/04/10 00:00 [revised]
PHST- 2008/06/22 00:00 [accepted]
PHST- 2008/07/22 09:00 [pubmed]
PHST- 2008/11/11 09:00 [medline]
PHST- 2008/07/22 09:00 [entrez]
AID - S1065-6995(08)00473-3 [pii]
AID - 10.1016/j.cellbi.2008.06.005 [doi]
PST - ppublish
SO  - Cell Biol Int. 2008 Sep;32(9):1150-7. doi: 10.1016/j.cellbi.2008.06.005. Epub 
      2008 Jun 28.