PMID- 18639596
OWN - NLM
STAT- MEDLINE
DCOM- 20090709
LR  - 20181201
IS  - 1873-4995 (Electronic)
IS  - 0168-3659 (Linking)
VI  - 132
IP  - 3
DP  - 2008 Dec 18
TI  - Dendritic multishell architectures for drug and dye transport.
PG  - 289-94
LID - 10.1016/j.jconrel.2008.06.016 [doi]
AB  - Here we present the efficiency and versatility of newly developed core-multishell 
      nanoparticles (CMS NPs), to encapsulate and transport the antitumor drugs 
      doxorubicin hydrochloride (Dox), methotrexate (Mtx) and sodium ibandronate (Ibn) 
      as well as dye molecules, i.e., a tetrasulfonated indotricarbocyanine (ITCC) and 
      nile red. Structurally, the CMS NPs are composed of hyperbranched poly(ethylene 
      imine) core functionalized by alkyl diacids connected to monomethyl poly(ethylene 
      glycol). In order to evaluate their transport in aqueous media in vitro, we have 
      used and compared SEC, UV, ITC, and NMR techniques. We observed that the CMS NPs 
      were able to spontaneously encapsulate and transport Dox, Mtx and nile red in 
      both organic and aqueous media as determined by SEC and UV-VIS spectroscopy. For 
      the VIS transparent Ibn Isothermal Titration Calorimetric (ITC) experiments show 
      an exothermic interaction with the CMS NPs. The enthalpic stabilization (DeltaH) 
      upon encapsulation was in the order of approximately 7 kcals/mol which indicates 
      stable interaction between Ibn and nanoparticles. A T(1) inversion recovery NMR 
      experiment was carried out for 31P and 1H nuclei of Ibn and an increment of 
      spin-lattice relaxation time for respective nuclei was observed upon 
      encapsulation. CMS NPs were also found to encapsulate ITCC dye with stoichiometry 
      of 6-8 molecules/nanocarrier. For in vivo imaging studies the dye loaded CMS NPs 
      were injected to F9 teratocarcinoma bearing mice and a strong contrast was 
      observed in the tumor tissues compared to free dye after 6 h of administration.
FAU - Quadir, Mohiuddin A
AU  - Quadir MA
AD  - Institut fur Chemie und Biochemie, Freie Universitat Berlin, Takustrasse 3, 14195 
      Berlin, Germany.
FAU - Radowski, Michal R
AU  - Radowski MR
FAU - Kratz, Felix
AU  - Kratz F
FAU - Licha, Kai
AU  - Licha K
FAU - Hauff, Peter
AU  - Hauff P
FAU - Haag, Rainer
AU  - Haag R
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20080623
PL  - Netherlands
TA  - J Control Release
JT  - Journal of controlled release : official journal of the Controlled Release 
      Society
JID - 8607908
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Carbocyanines)
RN  - 0 (Coloring Agents)
RN  - 0 (Diphosphonates)
RN  - 0 (Drug Carriers)
RN  - 0 (Oxazines)
RN  - 0 (indotricarbocyanine)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - 80168379AG (Doxorubicin)
RN  - 9002-98-6 (Polyethyleneimine)
RN  - P476F1L81G (nile red)
RN  - UMD7G2653W (Ibandronic Acid)
RN  - YL5FZ2Y5U1 (Methotrexate)
SB  - IM
MH  - Animals
MH  - Antineoplastic Agents/chemistry/*metabolism
MH  - Calorimetry
MH  - Carbocyanines/metabolism
MH  - Chemistry, Pharmaceutical
MH  - Chromatography, Gel
MH  - Coloring Agents/administration & dosage/chemistry/*metabolism/pharmacokinetics
MH  - Diphosphonates/metabolism
MH  - Doxorubicin/metabolism
MH  - *Drug Carriers
MH  - Drug Compounding
MH  - Ibandronic Acid
MH  - Injections, Intravenous
MH  - Magnetic Resonance Spectroscopy
MH  - Methotrexate/metabolism
MH  - Mice
MH  - *Nanoparticles
MH  - Oxazines/metabolism
MH  - Polyethylene Glycols/*chemistry
MH  - Polyethyleneimine/*chemistry
MH  - Spectrophotometry, Ultraviolet
MH  - Technology, Pharmaceutical/methods
MH  - Teratocarcinoma/metabolism
EDAT- 2008/07/22 09:00
MHDA- 2009/07/10 09:00
CRDT- 2008/07/22 09:00
PHST- 2008/04/29 00:00 [received]
PHST- 2008/06/13 00:00 [revised]
PHST- 2008/06/16 00:00 [accepted]
PHST- 2008/07/22 09:00 [pubmed]
PHST- 2009/07/10 09:00 [medline]
PHST- 2008/07/22 09:00 [entrez]
AID - S0168-3659(08)00345-3 [pii]
AID - 10.1016/j.jconrel.2008.06.016 [doi]
PST - ppublish
SO  - J Control Release. 2008 Dec 18;132(3):289-94. doi: 10.1016/j.jconrel.2008.06.016. 
      Epub 2008 Jun 23.