PMID- 18639615
OWN - NLM
STAT- MEDLINE
DCOM- 20081218
LR  - 20211020
IS  - 0306-4522 (Print)
IS  - 0306-4522 (Linking)
VI  - 155
IP  - 3
DP  - 2008 Aug 26
TI  - Coordinate action of pre- and postsynaptic brain-derived neurotrophic factor is 
      required for AMPAR trafficking and acquisition of in vitro classical 
      conditioning.
PG  - 686-97
LID - 10.1016/j.neuroscience.2008.06.043 [doi]
AB  - Brain-derived neurotrophic factor (BDNF) has been implicated in mechanisms of 
      synaptic plasticity such as long-term potentiation (LTP), but its role in 
      associative learning remains largely unknown. In the present study, we 
      investigated the function of BDNF and its receptor tropomyosin-related kinase B 
      (TrkB) in an in vitro model of classical conditioning using pond turtles, 
      Pseudemys scripta elegans. Conditioning resulted in a significant increase in 
      BDNF and phospho (p)-Trk expression. Bath application of antibodies directed 
      against TrkB, but not TrkA or TrkC, abolished acquisition of conditioning, as did 
      a receptor tyrosine kinase inhibitor K252a and an inhibitor of nitric oxide 
      synthase 7-nitroindazole. Significantly, injections of BDNF Ab into the nerve 
      roots of presynaptic axonal projections or postsynaptic motor neurons prevented 
      acquisition of conditioning, suggesting that BDNF is required on both sides of 
      the synapse for modification to occur. The presynaptic proteins synaptophysin and 
      synapsin I were increased upon conditioning or BDNF application. Furthermore, 
      BDNF application alone mimicked conditioning-induced synaptic insertion of GluR1 
      and GluR4 AMPAR subunits into synapses, which was inhibited by co-application of 
      BDNF and K252a. Data also show that extracellular signal-regulated kinase (ERK) 
      was activated in BDNF-treated preparations. We conclude that coordinate pre- and 
      postsynaptic actions of BDNF are required for acquisition of in vitro classical 
      conditioning.
FAU - Li, W
AU  - Li W
AD  - Neuroscience Group, Division of Basic Biomedical Sciences, University of South 
      Dakota School of Medicine, 414 East Clark Street, Vermillion, SD 57069, USA.
FAU - Keifer, J
AU  - Keifer J
LA  - eng
GR  - P20 RR-015567/RR/NCRR NIH HHS/United States
GR  - P20 RR015567-09/RR/NCRR NIH HHS/United States
GR  - P20 RR015567/RR/NCRR NIH HHS/United States
GR  - R01 NS051187-08/NS/NINDS NIH HHS/United States
GR  - R01 NS051187-09/NS/NINDS NIH HHS/United States
GR  - P20 RR015567-08/RR/NCRR NIH HHS/United States
GR  - NS-051187/NS/NINDS NIH HHS/United States
GR  - R01 NS051187/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20080625
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Antibodies)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Receptors, AMPA)
RN  - 452VLY9402 (Serine)
RN  - EC 2.7.10.1 (Receptor, trkA)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
SB  - IM
MH  - Animals
MH  - Antibodies/pharmacology
MH  - Brain Stem/cytology/physiology
MH  - Brain-Derived Neurotrophic Factor/immunology/*metabolism
MH  - Cochlear Nerve/physiology
MH  - Conditioning, Classical/*drug effects/physiology
MH  - Enzyme Inhibitors/pharmacology
MH  - Extracellular Signal-Regulated MAP Kinases/metabolism
MH  - Gene Expression Regulation/drug effects/physiology
MH  - In Vitro Techniques
MH  - Motor Neurons/cytology/drug effects/physiology
MH  - Neural Pathways/drug effects/metabolism
MH  - Phosphorylation
MH  - Physical Stimulation/methods
MH  - Protein Transport/drug effects
MH  - Receptor, trkA/metabolism
MH  - Receptors, AMPA/*metabolism
MH  - Serine/metabolism
MH  - Synapses/*drug effects
MH  - Time Factors
MH  - Turtles
PMC - PMC2607041
MID - NIHMS68807
EDAT- 2008/07/22 09:00
MHDA- 2008/12/19 09:00
PMCR- 2009/08/26
CRDT- 2008/07/22 09:00
PHST- 2008/04/03 00:00 [received]
PHST- 2008/05/23 00:00 [revised]
PHST- 2008/06/11 00:00 [accepted]
PHST- 2008/07/22 09:00 [pubmed]
PHST- 2008/12/19 09:00 [medline]
PHST- 2008/07/22 09:00 [entrez]
PHST- 2009/08/26 00:00 [pmc-release]
AID - S0306-4522(08)00901-9 [pii]
AID - 10.1016/j.neuroscience.2008.06.043 [doi]
PST - ppublish
SO  - Neuroscience. 2008 Aug 26;155(3):686-97. doi: 10.1016/j.neuroscience.2008.06.043. 
      Epub 2008 Jun 25.